Takumi Ishizuka

Last updated: Feb 25, 2019 at 17:30

Name	Takumi Ishizuka
Affiliation	University of Miyazaki
Section	Faculty of Medicine

Research Interests

Biotechnology(127) , Nucleic acid(22) , G-quadruplex(10) , Telomere(23) , RNA(81)

Research Areas

Applied chemistry / Bio-related chemistry /

Academic & Professional Experience

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Apr 2016

Today

Assistant Professor, Faculty of Medicine, University of Miyazaki

Jan 2013

Mar 2016

Project Assistant Professor, Faculty of Medicine, University of Miyazaki

Apr 2011

Dec 2012

The Japan Society for the Promotion of Science (JSPS) Research Fellow (Chemistry, PD), Systems and Structural Biology Center, RIKEN

Apr 2009

Mar 2011

The Japan Society for the Promotion of Science (JSPS) Research Fellow (Chemistry, DC2), Research Center for Advanced Science and Technology, The University of Tokyo

Education

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Mar 2011

Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo

Mar 2008

Department of Bioengineering, School of Engineering, The University of Tokyo

Mar 2006

School of Bioscience and Biotechnology, Tokyo Institute of Technology

Published Papers

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Stability and properties of Z-DNA containing artificial nucleobase 2'-O-methyl-8-methyl guanosine.

Balasubramaniyam T, Ishizuka T, Xu Y

Bioorganic & medicinal chemistry 27(2) 364-369 Jan 2019 [Refereed]

2′-O-Methyl-8-methylguanosine as a Z-Form RNA Stabilizer for Structural and Functional Study of Z-RNA

Thananjeyan Balasubramaniyam, Takumi Ishizuka, Chao-Da Xiao, Hong-Liang Bao, Yan Xu

Molecules 23(10) 2572 Oct 2018 [Refereed]

Studying DNA G-Quadruplex Aptamer by 19F NMR

Takumi Ishizuka, Atsushi Yamashita, Yujiro Asada, Yan Xu

ACS Omega 2(12) 8843-8848 Dec 2017 [Refereed]

A multi-functional guanine derivative for studying the DNA G-quadruplex structure.

Ishizuka T, Zhao PY, Bao HL, Xu Y

The Analyst 142(21) 4083-4088 Oct 2017 [Refereed]

Antiparallel RNA G-quadruplex Formed by Human Telomere RNA Containing 8-Bromoguanosine.

Xiao CD, Ishizuka T, Xu Y

Scientific reports 7(1) 6695 Jul 2017 [Refereed]

Structure-Dependent Binding of hnRNPA1 to Telomere RNA.

Liu X, Ishizuka T, Bao HL, Wada K, Takeda Y, Iida K, Nagasawa K, Yang D, Xu Y

Journal of the American Chemical Society 139(22) 7533-7539 Jun 2017 [Refereed]

Cover Picture: A Simple and Sensitive 19F NMR Approach for Studying the Interaction of RNA G-Quadruplex with Ligand Molecule and Protein (ChemistrySelect 15/2017)

Hong-Liang Bao, Takumi Ishizuka, Ayaka Iwanami, Takanori Oyoshi, Yan Xu

ChemistrySelect 2(15) 4169 May 2017 [Refereed]

A Simple and Sensitive 19F NMR Approach for Studying the Interaction of RNA G-Quadruplex with Ligand Molecule and Protein

Hong-Liang Bao, Takumi Ishizuka, Ayaka Iwanami, Takanori Oyoshi, Yan Xu

ChemistrySelect 2(15) 4170 May 2017 [Refereed]

Characterization of human telomere RNA G-quadruplex structures in vitro and in living cells using 19F NMR spectroscopy.

Bao HL, Ishizuka T, Sakamoto T, Fujimoto K, Uechi T, Kenmochi N, Xu Y

Nucleic acids research 45(9) 5501-5511 May 2017 [Refereed]

Unusual Topological RNA Architecture with an Eight-Stranded Helical Fragment Containing A-, G-, and U-Tetrads.

Xiao CD, Ishizuka T, Zhu XQ, Li Y, Sugiyama H, Xu Y

Journal of the American Chemical Society 139(7) 2565-2568 Feb 2017 [Refereed]

Click Chemistry Takes a Snapshot of DNA-RNA Hybrid G-Quadruplex in Living Cells

Nov 2016 [Refereed]

Click Chemistry Takes a Snapshot of DNA-RNA Hybrid G-Quadruplex in Living Cells

Ishizuka T, Xu Y

Journal of Nature and Science 2(10) e237 Oct 2016 [Refereed]

Fluorescence imaging of chromosomal DNA using click chemistry.

Ishizuka T, Liu HS, Ito K, Xu Y

Scientific reports 6 33217 Sep 2016 [Refereed]

Clipping of Telomere from Human Chromosomes Using a Chemistry-Based Artificial Restriction DNA Cutter.

Ishizuka T, Xu Y, Komiyama M

Current Protocols in Nucleic Acid Chemistry 61 6.13.1-6.13.13 Jun 2015 [Refereed]

Finding a human telomere DNA-RNA hybrid G-quadruplex formed by human telomeric 6-mer RNA and 16-mer DNA using click chemistry: a protective structure for telomere end.

Xu Y, Suzuki Y, Ishizuka T, Xiao CD, Liu X, Hayashi T, Komiyama M

Bioorganic & Medicinal Chemistry 22(16) 4419-4421 Aug 2014 [Refereed]

Conjugation of peptide nucleic acid with a pyrrole/imidazole polyamide to specifically recognize and cleave DNA.

Kameshima W, Ishizuka T, Minoshima M, Yamamoto M, Sugiyama H, Xu Y, Komiyama M

Angewandte Chemie International Edition 52(51) 13681-13684 Dec 2013 [Refereed]

A chemistry-based method to detect individual telomere length at a single chromosome terminus.

Ishizuka T, Xu Y, Komiyama M

Journal of the American Chemical Society 135(1) 14-17 Jan 2013 [Refereed]

Oligonucleotide models of telomeric DNA and RNA form a Hybrid G-quadruplex structure as a potential component of telomeres.

Xu Y, Ishizuka T, Yang J, Ito K, Katada H, Komiyama M, Hayashi T

The Journal of Biological Chemistry 287(50) 41787-41796 Dec 2012 [Refereed]

Site-specific functionalization of RNA molecules by an unnatural base pair transcription system via click chemistry.

Ishizuka T, Kimoto M, Sato A, Hirao I

Chemical Communications 48(88) 10835-10837 Nov 2012 [Refereed]

G-rich sequence-specific recognition and scission of human genome by PNA/DNA hybrid G-quadruplex formation.

Ishizuka T, Yang J, Komiyama M, Xu Y

Angewandte Chemie International Edition 51(29) 7198-7202 Jul 2012 [Refereed]

A U-tetrad stabilizes human telomeric RNA G-quadruplex structure.

Xu Y, Ishizuka T, Kimura T, Komiyama M

Journal of the American Chemical Society 132(21) 7231-7233 Jun 2010 [Refereed]

SSB-assisted duplex invasion of preorganized PNA into double-stranded DNA.

Ishizuka T, Tedeschi T, Corradini R, Komiyama M, Sforza S, Marchelli R

ChemBioChem 10(16) 2607-2612 Nov 2009 [Refereed]

Strand invasion of conventional PNA to arbitrary sequence in DNA assisted by single-stranded DNA binding protein.

Ishizuka T, Otani K, Sumaoka J, Komiyama M

Chemical Communications (10) 1225-1227 Mar 2009 [Refereed]

Origin of high fidelity in target-sequence recognition by PNA-Ce(IV)/EDTA combinations as site-selective DNA cutters.

Miyajima Y, Ishizuka T, Yamamoto Y, Sumaoka J, Komiyama M

Journal of the American Chemical Society 131(7) 2657-2662 Feb 2009 [Refereed]

Promotion of strand invasion by utilizing entropically-favored PNA.

Ishizuka T, Sforza S, Komiyama M

Nucleic Acids Symposium Series (53) 161-162 2009

Consecutive formation of G-quadruplexes in human telomeric-overhang DNA: a protective capping structure for telomere ends.

Xu Y, Ishizuka T, Kurabayashi K, Komiyama M

Angewandte Chemie International Edition 48(42) 7833-7836 2009 [Refereed]

Chiral introduction of positive charges to PNA for double-duplex invasion to versatile sequences.

Ishizuka T, Yoshida J, Yamamoto Y, Sumaoka J, Tedeschi T, Corradini R, Sforza S, Komiyama M

Nucleic Acids Research 36(5) 1464-1471 Mar 2008 [Refereed]

Solid-phase synthesis of pseudo-complementary peptide nucleic acids.

Komiyama M, Aiba Y, Ishizuka T, Sumaoka J

Nature Protocols 3(4) 646-654 2008 [Refereed]

Iron-catalyst-switched selective conjugate addition of grignard reagents: alpha,beta,gamma,delta-unsaturated amides as versatile templates for asymmetric three-component coupling processes.

Okada S, Arayama K, Murayama R, Ishizuka T, Hara K, Hirone N, Hata T, Urabe H

Angewandte Chemie International Edition 47(36) 6860-6864 2008 [Refereed]

Mismatch recognition in PNA double-duplex invasion.

Miyajima Y, Ishizuka T, Komiyama M

Nucleic Acids Symposium Series (52) 125-126 2008

Single-strand binding protein enhances invasion of a PNA strand to double-stranded DNA.

Ishizuka T, Komiyama M

Nucleic Acids Symposium Series (52) 141-142 2008

Misc

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パラコートとジクワットの医学教育

湯川修弘, 徐岩, 柿崎英二, 石塚匠, 落合秀信

法医学の実際と研究 (58) 237-249 Nov 2015

Association Memberships

Japanese Society for Photomedicine and Photobiology(8) , THE CHEMICAL SOCIETY OF JAPAN(2674)

Research Grants & Projects

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Ministry of Education, Culture, Sports, Science and Technology: Grant-in-Aid for Young Scientists (B)

Project Year: Apr 2016 - Mar 2018 Investigator(s): Takumi Ishizuka

Ministry of Education, Culture, Sports, Science and Technology: Grant-in-Aid for Research Activity Start-up

Project Year: Aug 2013 - Mar 2015 Investigator(s): Takumi Ishizuka