Glycation stress (glycative stress) is a general concept of biological stress caused by a series of non-enzymatic glycation reactions, including advanced glycation end products (AGEs) formation, AGEs accumulation, glycation-associated dysfunction of proteins and cellular signaling, inflammation, oxidation, and/or tissue damage. There has been increasing evidence supporting a profound effect of AGEs on human diseases such as type 2 diabetes, cardiovascular disease, cancer, Alzheimer’s disease, osteoporosis, and dementia, as well as aging process itself. In addition, dietary AGEs intake has also been suggested to contribute to tissue dysfunction and development of the diseases. Skeletal muscle is the largest organ in the human body and important responsibility for maintaining our health as not only locomotor system but also metabolic and endocrine systems. Especially in past decades, numerous studies have suggested the contribution of glycation stress to skeletal muscle dysfunctions (e.g. muscle atrophy, reducing contractile property, and insulin resistance). In this chapter, we provide current evidence on the potential role of glycation stress in the impairment of skeletal muscle functions.