2012年12月14日
Different Ca2+-sensitivities between the EF-hands of T- and L-plastins
Biochemical and Biophysical Research Communications
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- 巻
- 429
- 号
- 3-4
- 開始ページ
- 137
- 終了ページ
- 141
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.bbrc.2012.10.126
- 出版者・発行元
- ACADEMIC PRESS INC ELSEVIER SCIENCE
Plastins are Ca2+-regulated actin-bundling proteins, and essential for developing and stabilizing actin cytoskeletons. T-plastin is expressed in epithelial and mesenchymal cells of solid tissues, whereas L-plastin is expressed in mobile cells such as hemopoietic cell lineages and cancer cells.
Using various spectroscopic methods, gel-filtration chromatography, and isothermal titration calorimetry, we here demonstrate that the EF-hand motifs of both T- and L-plastin change their structures in response to Ca2+, but the sensitivity to Ca2+ is lower in T-plastin than in L-plastin. These results suggest that T-plastin is suitable for maintaining static cytoskeletons, whereas L-plastin is suitable for dynamic rearrangement of cytoskeletons. (C) 2012 Elsevier Inc. All rights reserved.
Using various spectroscopic methods, gel-filtration chromatography, and isothermal titration calorimetry, we here demonstrate that the EF-hand motifs of both T- and L-plastin change their structures in response to Ca2+, but the sensitivity to Ca2+ is lower in T-plastin than in L-plastin. These results suggest that T-plastin is suitable for maintaining static cytoskeletons, whereas L-plastin is suitable for dynamic rearrangement of cytoskeletons. (C) 2012 Elsevier Inc. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.bbrc.2012.10.126
- ISSN : 0006-291X
- PubMed ID : 23142595
- Web of Science ID : WOS:000312514200003