Papers

Peer-reviewed
10 2017

Cholesterol concentrations in lipoprotein fractions separated by anion-exchange-high-performance liquid chromatography in healthy dogs and dogs with hypercholesterolemia

RESEARCH IN VETERINARY SCIENCE
  • Hitomi Oda
  • ,
  • Akihiro Mori
  • ,
  • Yuji Hirowatari
  • ,
  • Toshie Takoura
  • ,
  • Daisuke Manita
  • ,
  • Tomoya Takahashi
  • ,
  • Saori Shono
  • ,
  • Eri Onozawa
  • ,
  • Hisashi Mizutani
  • ,
  • Yohei Miki
  • ,
  • Yukiko Itabashi
  • ,
  • Toshinori Sako

Volume
114
Number
First page
163
Last page
169
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1016/j.rvsc.2017.04.004
Publisher
ELSEVIER SCI LTD

Anion-exchange (AEX)-high-performance liquid chromatography (HPLC) for measurement of cholesterol can be used to separate serum lipoproteins (high-density lipoprotein (HDL); low-density lipoprotein (LDL); intermediate-density lipoprotein (IDL); very-low-density lipoprotein (VLDL)) in humans. However, AEX-HPLC has not been applied in veterinary practice. We had three objectives: (i) the validation of AEX-HPLC methods including the correlation of serum cholesterol concentration in lipoprotein fraction measured by AEX-HPLC and gel permeation-HPLC (GP-HPLC) in healthy dogs and those with hypercholesterolemia was investigated; (ii) the reference intervals of lipoprotein fractions measured by AEX-HPLC from healthy dogs (n = 40) was established; (iii) lipoprotein fractions from the serum of healthy dogs (n = 12) and dogs with hypercholesterolemia (n = 23) were compared. Analytic reproducibility and precision of AEX-HPLC were acceptable. Positive correlation between serum concentrations of total cholesterol (Total-Chol), HDL cholesterol (HDL-Chol), LDL cholesterol (LDL-Chol) + IDL cholesterol (IDL-Chol), and VLDL cholesterol (VLDL-Chol) was noted for AEX-HPLC and GP-HPLC in healthy dogs and dogs with hypercholesterolemia. Reference intervals measured by AEX-HPLC for serum concentrations of Total-Chol, HDL-Chol, and LDL-Chol were determined to be 2.97-9.32, 2.79-6.57, 0.16-3.28 mmol/L (2.5-97.5% interval), respectively. Furthermore, there was significant difference in lipoprotein profiles between healthy and dogs with hypercholesterolemia. These results suggest that AEX-HPLC can be used to evaluate lipoprotein profiles in dogs and could be a new useful indicator of hyperlipidemia in dogs.

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Link information
DOI
https://doi.org/10.1016/j.rvsc.2017.04.004
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28419940
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000414881800026&DestApp=WOS_CPL

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