2012年1月
Heavy metal-free F-19 NMR probes for quantitative measurements of glutathione reductase activity using silica nanoparticles as a signal quencher
BIOORGANIC & MEDICINAL CHEMISTRY
- ,
- ,
- 巻
- 20
- 号
- 1
- 開始ページ
- 96
- 終了ページ
- 100
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.bmc.2011.11.026
- 出版者・発行元
- PERGAMON-ELSEVIER SCIENCE LTD
For the quantitative assessment of the glutathione reductase (GR) activity with a F-19 NMR spectroscopy, we developed the heavy metal-free probes based on silica nanoparticles modified with water-soluble perfluorinated dendrimers via the disulfide linkers. Before enzymatic reaction, the molecular rotation of the perfluorinated dendrimers is highly restricted, and the magnitude of F-19 NMR signals from the perfluorinated dendrimers can be suppressed. By the reductive cleavage of the disulfide linkers with the reduced glutathione-mediated enzymatic reaction of GR, perfluorinated dendrimers can be released from the surfaces of the nanoparticles. Consequently, the F-19 NMR signals of perfluorinated dendrimers were recovered. The enzymatic activity of GR was determined from the increase of the magnitude of F-19 NMR signals. Finally, to demonstrate the feasibility of the probe in the presence of miscellaneous molecules under bio-mimetic conditions, the comparison study was executed with the cancer cell lysate. The value determined from our method showed a good agreement with that from the conventional method. (C) 2011 Elsevier Ltd. All rights reserved.
- リンク情報
- ID情報
-
- DOI : 10.1016/j.bmc.2011.11.026
- ISSN : 0968-0896
- Web of Science ID : WOS:000298633300009