2009年6月
Preparation and fluorescence properties of fluorophore-labeled avidin-biotin system immobilized on Fe3O4 nanoparticles through functional indolequinone linker
BIOORGANIC & MEDICINAL CHEMISTRY
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- 巻
- 17
- 号
- 11
- 開始ページ
- 3775
- 終了ページ
- 3781
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.bmc.2009.04.048
- 出版者・発行元
- PERGAMON-ELSEVIER SCIENCE LTD
We prepared and characterized a new class of fluorophore-labeled magnetic nanoparticles (MNPs) possessing a hypoxia-responsive unit to construct a hypoxia-selective emission system. The indolequinone derivative as a hypoxia-response unit bearing biotin was synthesized and immobilized on Fe3O4 MNP. Subsequent complexation of this functionalized MNP with fluorescein-labeled avidin formed fluorophore-labeled nanoparticles (AF-QB@MNP). The fluorescence intensity of AF-QB@MNP was suppressed because of the adjacent quenching function of the indolequinone moiety and MNP. Upon hypoxic treatment by NADPH: cytochrome P450 reductase, AF-QB@MNP was activated to liberate a fluorescence unit, leading to the significant enhancement of fluorescence emission, while a smaller enhancement in fluorescence emission occurred upon aerobic treatment. The AF-QB@MNP has a indispensable properties as a fluorescent probe for imaging of disease relevant hypoxic microenvironments. (C) 2009 Elsevier Ltd. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.bmc.2009.04.048
- ISSN : 0968-0896
- eISSN : 1464-3391
- Web of Science ID : WOS:000266117400004