2004年9月
A novel nucleolar protein, PAPA-1, induces growth arrest as a result of cell cycle arrest at the G1 phase
GENE
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- 巻
- 340
- 号
- 1
- 開始ページ
- 83
- 終了ページ
- 98
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.gene.2004.05.025
- 出版者・発行元
- ELSEVIER SCIENCE BV
We have identified a novel nucleolar protein, PAP-1-associated protein-1 (PAPA-1), after screening the interacting proteins with Pim-1-associated protein-1 (PAP-1), a protein that is a phosphorylation target of Pim-1 kinase. PAPA-1 comprises 345 amino acids with a basic amino-acid cluster. PAPA-1 was found to be localized in the nucleolus in transfected HeLa cells, and the lysine/histidine cluster was essential for nucleolar localization of PAPA-1. PAPA-1 protein and mRNA expression decreased upon serum restimulation of starvation-synchronized cells, which displayed maximum level of PAPA-1 expression at G0 and early G1 phase of the cell cycle. Ectopic expression of PAPA-1 induced growth suppression of cells, and the effect was dependent on its nucleolar localization in established HeLa cell lines that inducibly express PAPA-1 or its deletion mutant under the control of a tetracycline-inducible promoter. Furthermore, when PAPA-1-inducible HeLa cells were synchronized by thymidine, colcemid or mimosine, and then PAPA-1 was expressed, the proportion of cells at the G1 phase was obviously increased. These results suggest that PAPA-1 induces growth and cell cycle arrests at the G1 phase of the cell cycle. (C) 2004 Elsevier B.V. All rights reserved.
- リンク情報
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- DOI
- https://doi.org/10.1016/j.gene.2004.05.025
- CiNii Articles
- http://ci.nii.ac.jp/naid/80016920016
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/15556297
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000224359500009&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1016/j.gene.2004.05.025
- ISSN : 0378-1119
- CiNii Articles ID : 80016920016
- PubMed ID : 15556297
- Web of Science ID : WOS:000224359500009