論文

査読有り
2017年5月

Mucosal IgM Antibody with D-Mannose Affinity in Fugu Takifugu rubripes Is Utilized by a Monogenean Parasite Heterobothrium okamotoi for Host Recognition

JOURNAL OF IMMUNOLOGY
  • Kento Igarashi
  • ,
  • Ryohei Matsunaga
  • ,
  • Sachi Hirakawa
  • ,
  • Sho Hosoya
  • ,
  • Hiroaki Suetake
  • ,
  • Kiyoshi Kikuchi
  • ,
  • Yuzuru Suzuki
  • ,
  • Osamu Nakamura
  • ,
  • Toshiaki Miyadai
  • ,
  • Satoshi Tasumi
  • ,
  • Shigeyuki Tsutsui

198
10
開始ページ
4107
終了ページ
4114
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.4049/jimmunol.1601996
出版者・発行元
AMER ASSOC IMMUNOLOGISTS

How parasites recognize their definitive hosts is a mystery; however, parasitism is reportedly initiated by recognition of certain molecules on host surfaces. Fish ectoparasites make initial contact with their hosts at body surfaces, such as skin and gills, which are covered with mucosa that are similar to those of mammalian guts. Fish are among the most primitive vertebrates with immune systems that are equivalent to those in mammals, and they produce and secrete IgM into mucus. In this study, we showed that the monogenean parasite Heterobothrium okamotoi utilizes IgM to recognize its host, fugu Takifugu rubripes. Oncomiracidia are infective larvae of H. okamotoi that shed their cilia and metamorphose into juveniles when exposed to purified D-mannose-binding fractions from fugu mucus. Using liquid chromatography-tandem mass spectrometry analysis, proteins contained in the fraction were identified as D-mannose-specific IgM with two D-mannose-binding lectins. However, although deciliation was significantly induced by IgM and was inhibited by D-mannose or a specific Ab against fugu IgM, other lectins had no effect, and IgM without D-mannose affinity induced deciliation to a limited degree. Subsequent immunofluorescent staining experiments showed that fugu D-mannose-specific IgM binds ciliated epidermal cells of oncomiracidium. These observations suggest that deciliation is triggered by binding of fugu IgM to cell surface Ags via Ag binding sites. Moreover, concentrations of D-mannose-binding IgM in gill mucus were sufficient to induce deciliation in vitro, indicating that H. okamotoi parasites initially use host Abs to colonize host gills.

Web of Science ® 被引用回数 : 4

リンク情報
DOI
https://doi.org/10.4049/jimmunol.1601996
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28404634
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000401137200033&DestApp=WOS_CPL
URL
http://europepmc.org/abstract/med/28404634
URL
http://orcid.org/0000-0003-3047-6942