論文

査読有り
2016年6月

Identification and characterization of pufflectin from the grass pufferfish Takifugu niphobles and comparison of its expression with that of Takifugu rubripes

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
  • Satoshi Tasumi
  • ,
  • Akira Yamaguchi
  • ,
  • Ryohei Matsunaga
  • ,
  • Kazuma Fukushi
  • ,
  • Yuzuru Suzuki
  • ,
  • Osamu Nakamura
  • ,
  • Kiyhoshi Kikuchi
  • ,
  • Shigeyuki Tsutsui

59
開始ページ
48
終了ページ
56
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.dci.2016.01.007
出版者・発行元
ELSEVIER SCI LTD

Pufflectin found in Takifugu rubripes (Tr pufflectin) is the first animal lectin reported to show sequence similarity to monocotyledonous plant lectins. In the present study, we identified and characterized an orthologous lectin from Takifugu niphobles (Tn pufflectin), a species closely related to T. rubripes. Tn pufflectin exhibits 86% identity to Tr pufflectin with two conserved mannose-binding domains. Tn pufflectin was mainly expressed in the skin, gills, brain, and muscles; however, it was expressed at a lower level in the other examined tissues. Recombinant Tn pufflectin, expressed by Escherichia coli, exhibited binding activity specific for D-mannose. The expression of pufflectin in the gills was much lower in T niphobles than in T rubripes; notably, the former and latter are resistant and susceptible, respectively, to the monogenean parasite Heterobothrium okamotoi, which parasitizes gills. This suggests that pufflectin might be utilized by the parasite for host recognition. (C) 2016 Elsevier Ltd. All rights reserved.

Web of Science ® 被引用回数 : 6

リンク情報
DOI
https://doi.org/10.1016/j.dci.2016.01.007
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000373547000006&DestApp=WOS_CPL
URL
http://orcid.org/0000-0003-3047-6942