論文

査読有り 国際誌
2019年11月26日

Chondroitin sulfate E blocks enzymatic action of heparanase and heparanase-induced cellular responses.

Biochemical and biophysical research communications
  • Nobuaki Higashi
  • Rino Maeda
  • Nakaba Sesoko
  • Momoko Isono
  • Sodai Ishikawa
  • Yurina Tani
  • Katsuhiko Takahashi
  • Teruaki Oku
  • Kyohei Higashi
  • Shoichi Onishi
  • Motowo Nakajima
  • Tatsuro Irimura
  • 全て表示

520
1
開始ページ
152
終了ページ
158
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bbrc.2019.09.126

We examined whether chondroitin sulfates (CSs) exert inhibitory effects on heparanase (Hpse), the sole endoglycosidase that cleaves heparan sulfate (HS) and heparin, which also stimulates chemokine production. Hpse-mediated degradation of HS was suppressed in the presence of glycosaminoglycans derived from a squid cartilage and mouse bone marrow-derived mast cells, including the E unit of CS. Pretreatment of the chondroitin sulfate E (CS-E) with chondroitinase ABC abolished the inhibitory effect. Recombinant proteins that mimic pro-form and mature-form Hpse bound to the immobilized CS-E. Cellular responses as a result of Hpse-mediated binding, namely, uptake of Hpse by mast cells and Hpse-induced release of chemokine CCL2 from colon carcinoma cells, were also blocked by the CS-E. CS-E may regulate endogenous Hpse-mediated cellular functions by inhibiting enzymatic activity and binding to the cell surface.

リンク情報
DOI
https://doi.org/10.1016/j.bbrc.2019.09.126
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31582210
ID情報
  • DOI : 10.1016/j.bbrc.2019.09.126
  • PubMed ID : 31582210

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