論文

国際誌
2011年6月

Natural killer cells recognize friend retrovirus-infected erythroid progenitor cells through NKG2D-RAE-1 interactions In Vivo.

Journal of virology
  • Tatsuya Ogawa
  • ,
  • Sachiyo Tsuji-Kawahara
  • ,
  • Takae Yuasa
  • ,
  • Saori Kinoshita
  • ,
  • Tomomi Chikaishi
  • ,
  • Shiki Takamura
  • ,
  • Haruo Matsumura
  • ,
  • Tsukasa Seya
  • ,
  • Toshihiko Saga
  • ,
  • Masaaki Miyazawa

85
11
開始ページ
5423
終了ページ
35
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1128/JVI.02146-10

Natural killer (NK) cells function as early effector cells in the innate immune defense against viral infections and also participate in the regulation of normal and malignant hematopoiesis. NK cell activities have been associated with early clearance of viremia in experimental simian immunodeficiency virus and clinical human immunodeficiency virus type 1 (HIV-1) infections. We have previously shown that NK cells function as major cytotoxic effector cells in vaccine-induced immune protection against Friend virus (FV)-induced leukemia, and NK cell depletion totally abrogates the above protective immunity. However, how NK cells recognize retrovirus-infected cells remains largely unclear. The present study demonstrates a correlation between the expression of the products of retinoic acid early transcript-1 (RAE-1) genes in target cells and their susceptibility to killing by NK cells isolated from FV-infected animals. This killing was abrogated by antibodies blocking the NKG2D receptor in vitro. Further, the expression of RAE-1 proteins on erythroblast surfaces increased early after FV inoculation, and administration of an RAE-1-blocking antibody resulted in increased spleen infectious centers and exaggerated pathology, indicating that FV-infected erythroid cells are recognized by NK cells mainly through the NKG2D-RAE-1 interactions in vivo. Enhanced retroviral replication due to host gene-targeting resulted in markedly increased RAE-1 expression in the absence of massive erythroid cell proliferation, indicating a direct role of retroviral replication in RAE-1 upregulation.

リンク情報
DOI
https://doi.org/10.1128/JVI.02146-10
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/21411527
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3094956
ID情報
  • DOI : 10.1128/JVI.02146-10
  • PubMed ID : 21411527
  • PubMed Central 記事ID : PMC3094956

エクスポート
BibTeX RIS