論文

査読有り 責任著者
2017年8月

Severe developmental timing defects in the prothoracicotropic hormone (PTTH)-deficient silkworm, Bombyx mori

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
  • Miwa Uchibori-Asano
  • ,
  • Takumi Kayukawa
  • ,
  • Hideki Sezutsu
  • ,
  • Tetsuro Shinoda
  • ,
  • Takaaki Daimon

87
開始ページ
14
終了ページ
25
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.ibmb.2017.06.007
出版者・発行元
PERGAMON-ELSEVIER SCIENCE LTD

The insect neuropeptide prothoracicotropic hormone (PTTH) triggers the biosynthesis and release of the molting hormone ecdysone in the prothoracic gland (PG), thereby controlling the timing of molting and metamorphosis. Despite the well-documented physiological role of PTTH and its signaling pathway in the PG, it is not clear whether PTTH is an essential hormone for ecdysone biosynthesis and development. To address this question, we established and characterized a PTTH knockout line in the silkworm, Bombyx mori. We found that PTTH knockouts showed a severe developmental delay in both the larval and pupal stages. Larval phenotypes of PTTH knockouts can be classified into three major classes: (i) developmental arrest during the second larval instar, (ii) precocious metamorphosis after the fourth larval instar (one instar earlier in comparison to the control strain), and (iii) metamorphosis to normal-sized pupae after completing the five larval instar stages. In PTTH knockout larvae, peak levels of ecdysone titers in the hemolymph were dramatically reduced and the timing of peaks was delayed, suggesting that protracted larval development is a result of the reduced and delayed synthesis of ecdysone in the PG. Despite these defects, low basal levels of ecdysone were maintained in PTTH knockout larvae, suggesting that the primary role of PTTH is to upregulate ecdysone biosynthesis in the PG during molting stages, and low basal levels of ecdysone can be maintained in the absence of PTTH. We also found that mRNA levels of genes involved in ecdysone biosynthesis and ecdysteroid signaling pathways were significantly reduced in PTTH knockouts. Our results provide genetic evidence that PTTH is not essential for development, but is required to coordinate growth and developmental timing. (C) 2017 Elsevier Ltd. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.ibmb.2017.06.007
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000408289200002&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.ibmb.2017.06.007
  • ISSN : 0965-1748
  • eISSN : 1879-0240
  • Web of Science ID : WOS:000408289200002

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