論文

査読有り 国際誌
2018年9月21日

Impact of Dental Pulp Stem Cells Overexpressing Hepatocyte Growth Factor after Cerebral Ischemia/Reperfusion in Rats.

Molecular therapy. Methods & clinical development
  • Kota Sowa
  • ,
  • Chikako Nito
  • ,
  • Masataka Nakajima
  • ,
  • Satoshi Suda
  • ,
  • Yasuhiro Nishiyama
  • ,
  • Yuki Sakamoto
  • ,
  • Yuko Nitahara-Kasahara
  • ,
  • Aki Nakamura-Takahashi
  • ,
  • Masayuki Ueda
  • ,
  • Kazumi Kimura
  • ,
  • Takashi Okada

10
開始ページ
281
終了ページ
290
記述言語
英語
掲載種別
DOI
10.1016/j.omtm.2018.07.009

Hepatocyte growth factor (HGF) has neuroprotective effects against ischemia-induced injuries. Dental pulp stem cell (DPSC) transplantation attenuates tissue injury in the brain of rats with post-transient middle cerebral artery occlusion. We sought to determine whether DPSCs that overexpress HGF can enhance their therapeutic effects on brain damage post-ischemia/reperfusion injury. Treatment with DPSCs overexpressing HGF reduced infarct volumes compared to unmodified DPSC treatment at 3 and 7 days post-transient middle cerebral artery occlusion. The use of unmodified DPSCs and DPSCs overexpressing HGF was associated with improved motor function compared to that with administration of vehicle at 7 days post-transient middle cerebral artery occlusion. DPSCs overexpressing HGF significantly inhibited microglial activation and pro-inflammatory cytokine production along with suppression of neuronal degeneration. Post-reperfusion, DPSCs overexpressing HGF attenuated the decreases in tight junction proteins, maintained blood-brain barrier integrity, and increased microvessel density in peri-infarct areas. The administration of DPSCs overexpressing HGF during the acute phase of stroke increased their neuroprotective effects by modulating inflammation and blood-brain barrier permeability, thereby promoting improvements in post-ischemia/reperfusion brain injury.

リンク情報
DOI
https://doi.org/10.1016/j.omtm.2018.07.009
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30151417
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6108066

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