論文

査読有り
2016年9月25日

Functional roles of NOD1 in odontoblasts on dental pulp innate immunity

BioMed Research International
  • Yuki Hosokawa
  • ,
  • Kouji Hirao
  • ,
  • Hiromichi Yumoto
  • ,
  • Ayako Washio
  • ,
  • Tadashi Nakanishi
  • ,
  • Daisuke Takegawa
  • ,
  • Chiaki Kitamura
  • ,
  • Takashi Matsuo

Vol.2016
開始ページ
9325436
終了ページ
9325436
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1155/2016/9325436

Caries-related pathogens are first recognized by odontoblasts and induce inflammatory events that develop to pulpitis. Generally, initial sensing of microbial pathogens is mediated by pattern recognition receptors, such as Toll-like receptor and nucleotide-binding oligomerization domain (NOD); however, little is known about NODs in odontoblasts. In this study, the levels of NODs expressed in rat odontoblastic cell line, KN-3, were assessed by flow cytometry and the levels of chemokines in NOD-specific ligand-stimulated KN-3 cells were analyzed by real-time PCR and ELISA. The signal transduction pathway activated with NOD-specific ligand was assessed by blocking assay with specific inhibitors and reporter assay. In KN-3 cells, the expression level of NOD1 was stronger than that of NOD2 and the production of chemokines, such as CINC-1, CINC-2, CCL20, and MCP-1, was upregulated by stimulation with NOD1-specific ligand, but not with NOD2-specific ligand. CINC-2 and CCL20 production by stimulation with NOD1-specific ligand was reduced by p38 MAPK and AP-1 signaling inhibitors. Furthermore, the reporter assay demonstrated AP-1 activation in NOD1-specific ligand-stimulated KN-3 cells. These findings indicated that NOD1 expressed in odontoblasts functions to upregulate the chemokines expression via p38-AP-1 signaling pathway and suggested that NOD1 may play important roles in the initiation and progression of pulpitis.

リンク情報
DOI
https://doi.org/10.1155/2016/9325436
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27747243