論文

査読有り
2021年6月8日

Gene Expression Profiles of Human Cerebral Organoids Identify PPAR Pathway and PKM2 as Key Markers for Oxygen-Glucose Deprivation and Reoxygenation

Frontiers in Cellular Neuroscience
  • Naoki Iwasa
  • ,
  • Takeshi K. Matsui
  • ,
  • Naohiko Iguchi
  • ,
  • Kaoru Kinugawa
  • ,
  • Naritaka Morikawa
  • ,
  • Yoshihiko M. Sakaguchi
  • ,
  • Tomo Shiota
  • ,
  • Shinko Kobashigawa
  • ,
  • Mari Nakanishi
  • ,
  • Masaya Matsubayashi
  • ,
  • Riko Nagata
  • ,
  • Sotaro Kikuchi
  • ,
  • Tatsuhide Tanaka
  • ,
  • Nobuyuki Eura
  • ,
  • Takao Kiriyama
  • ,
  • Tesseki Izumi
  • ,
  • Kozue Saito
  • ,
  • Hiroshi Kataoka
  • ,
  • Yuichi Saito
  • ,
  • Wataru Kimura
  • ,
  • Akio Wanaka
  • ,
  • Yuhei Nishimura
  • ,
  • Eiichiro Mori
  • ,
  • Kazuma Sugie

15
記述言語
掲載種別
研究論文(学術雑誌)
DOI
10.3389/fncel.2021.605030
出版者・発行元
Frontiers Media SA

Ischemic stroke is one of the most common neurological diseases. However, the impact of ischemic stroke on human cerebral tissue remains largely unknown due to a lack of ischemic human brain samples. In this study, we applied cerebral organoids derived from human induced pluripotent stem cells to evaluate the effect of oxygen-glucose deprivation/reoxygenation (OGD/R). Pathway analysis showed the relationships between vitamin digestion and absorption, fat digestion and absorption, peroxisome proliferator-activated receptor (PPAR) signaling pathway, and complement and coagulation cascades. Combinational verification with transcriptome and gene expression analysis of different cell types revealed fatty acids-related PPAR signaling pathway and pyruvate kinase isoform M2 (<italic>PKM2</italic>) as key markers of neuronal cells in response to OGD/R. These findings suggest that, although there remain some limitations to be improved, our ischemic stroke model using human cerebral organoids would be a potentially useful tool when combined with other conventional two-dimensional (2D) mono-culture systems.

リンク情報
DOI
https://doi.org/10.3389/fncel.2021.605030
URL
https://www.frontiersin.org/articles/10.3389/fncel.2021.605030/full
ID情報
  • DOI : 10.3389/fncel.2021.605030
  • eISSN : 1662-5102

エクスポート
BibTeX RIS