2010年8月
Highly sensitive and specific analysis of sterol profiles in biological samples by HPLC-ESI-MS/MS
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
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- 巻
- 121
- 号
- 3-5
- 開始ページ
- 556
- 終了ページ
- 564
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.jsbmb.2010.03.030
- 出版者・発行元
- PERGAMON-ELSEVIER SCIENCE LTD
High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) is a powerful method for the microanalysis of compounds in biological samples. Compared with gas chromatography-mass spectrometry (GC-MS), this method is more broadly applicable to various compounds and usually does not require a derivatization step before analysis. However, when neutral sterols are analyzed, the sensitivities of usual HPLC-MS/MS method are not superior to those of GC-MS because the sterols are relatively resistant to ionization. In this review, we introduce the recent development of HPLC-MS/MS analysis for the quantification of non-cholesterol sterols. By adding an effective derivatization step to the conventional procedure, sterol analysis by HPLC-MS/MS surpassed that obtained by GC-MS in sensitivity. In addition, sufficient specificity of this method was achieved by selected reaction monitoring (SRM) and thorough chromatographic separation of each sterol. (C) 2010 Elsevier Ltd. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.jsbmb.2010.03.030
- ISSN : 0960-0760
- PubMed ID : 20302936
- Web of Science ID : WOS:000281174000011