2013年12月
Induction of Female-to-Male Sex Change in Adult Zebrafish by Aromatase Inhibitor Treatment
SCIENTIFIC REPORTS
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- 巻
- 3
- 号
- 開始ページ
- 3400
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/srep03400
- 出版者・発行元
- NATURE PUBLISHING GROUP
This study investigated whether undifferentiated germ and/or somatic stem cells remain in the differentiated ovary of a species that does not undergo sex changes under natural conditions and retain their sexual plasticity. The effect of aromatase inhibitor (AI)-treatment on sexually mature female zebrafish was examined. A 5-month AI treatment caused retraction of the ovaries after which testes-like organs appeared, and cyst structures filled with spermatozoa-like cells were observed in sections of these tissues. Electron microscopic observations revealed that these cells appeared as large sperm heads without tails. Sperm formation was re-examined after changing the diet to an AI-free food. A large number of normal sperm were obtained after eight weeks, and no formation of ovarian tissue was observed. Artificial fertilization using sperm from the sex-changed females was successful. These results demonstrated that sex plasticity remains in the mature ovaries of this species.
- リンク情報
- ID情報
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- DOI : 10.1038/srep03400
- ISSN : 2045-2322
- PubMed ID : 24292399
- Web of Science ID : WOS:000327783800009