2009年5月
Imaging renal structures by X-ray phase-contrast microtomography
KIDNEY INTERNATIONAL
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- 巻
- 75
- 号
- 9
- 開始ページ
- 945
- 終了ページ
- 951
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/ki.2009.42
- 出版者・発行元
- NATURE PUBLISHING GROUP
X-ray crystal interferometer-based X-ray phase-contrast microtomography (phase-contrast microtomography) is able to image microstructures within soft tissue without the use of a contrast agent. Here we determined the feasibility of using this technique in the non-destructive inspection of formalin-fixed kidney tissue from certain hamsters that spontaneously develop mesangial thickening with focal and segmental glomerulosclerosis, and from age-matched Syrian hamsters. We used a triple Laue-case X-ray interferometer with a 40 mu m-thick analyzer, a sample cell, and an X-ray charge-coupled-device camera with a 4.34 mu m pixel size. Images of glomeruli and tubular structures were similar to those seen using 40-100 x magnification on an optical microscope. In samples from two female glomerulosclerotic hamsters, seven scattered lesions were detected. The wedge-shaped pathological lesions included mild atrophic tubular walls, markedly dilated tubular lumen, high-density glomeruli, and widening of Bowman's space. The microvasculature was distinctly visualized in the specimens without any contrast agents. Hence, phase-contrast microtomography can detect small scattered lesions in diseased kidney tissue and is a powerful auxiliary tool for pre-histological evaluations.
- リンク情報
- ID情報
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- DOI : 10.1038/ki.2009.42
- ISSN : 0085-2538
- eISSN : 1523-1755
- Web of Science ID : WOS:000265220900012