2018年10月15日
Well-designed dopamine-imprinted polymer interface for selective and quantitative dopamine detection among catecholamines using a potentiometric biosensor
Biosensors and Bioelectronics
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- 巻
- 117
- 号
- 開始ページ
- 810
- 終了ページ
- 817
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.bios.2018.07.014
- 出版者・発行元
- ELSEVIER ADVANCED TECHNOLOGY
© 2018 Elsevier B.V. We report a well-designed biointerface enabling the selective and quantitative detection of dopamine (DA) using a potentiometric biosensor. To enhance the detection selectivity of DA, a DA-templated molecularly imprinted polymer (DA–MIP) was synthesized on the extended Au gate electrode of a field-effect transistor (FET) biosensor. For a quantitative DA analysis, the thickness of the DA–MIP was controlled to ca. 60 nm by surface-initiated atom transfer radical polymerization (SI-ATRP). In this process, the DA–MIP was copolymerized with vinylphenylboronic acid (vinyl-PBA), inducing molecular charges at the biointerface of the FET gate electrode. These charges were generated by the diol-binding between PBA and dopamine (a catecholamine), and were directly detected as a change in surface potential. In fact, the surface potential at the gate of the DA–MIP-coated FET responded significantly to DA added at concentrations ranging from 40 nM to 20 μM, whereas that of a non-imprinted polymer (NIP)-coated FET hardly changed over this range. Moreover, by measuring the kinetic parameters and electrochemical properties of well-designed devices with various added catecholamines, we confirmed that the DA–MIP-coated FET biosensor selectively and quantitatively detects DA.
- リンク情報
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- DOI
- https://doi.org/10.1016/j.bios.2018.07.014
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/30096735
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000442191900100&DestApp=WOS_CPL
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85049968531&origin=inward
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85049968531&origin=inward
- URL
- http://www.sciencedirect.com/science/article/pii/S0956566318305141
- URL
- http://orcid.org/0000-0001-6121-9274
- ID情報
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- DOI : 10.1016/j.bios.2018.07.014
- ISSN : 0956-5663
- eISSN : 1873-4235
- ORCIDのPut Code : 46688626
- PubMed ID : 30096735
- SCOPUS ID : 85049968531
- Web of Science ID : WOS:000442191900100