2004年1月
Importance of TRF1 for functional telomere structure
JOURNAL OF BIOLOGICAL CHEMISTRY
- ,
- ,
- ,
- 巻
- 279
- 号
- 2
- 開始ページ
- 1442
- 終了ページ
- 1448
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1074/jbc.M309138200
- 出版者・発行元
- AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Telomeres are comprised of telomeric DNA sequences and associated binding molecules. Their structure functions to protect the ends of linear chromosomes and ensure chromosomal stability. One of the mammalian telomere-binding factors, TRF1, localizes telomeres by binding to double-stranded telomeric DNA arrays. Because the overexpression of wild-type and dominant-negative TRF1 induces progressive telomere shortening and elongation in human cells, respectively, a proposed major role of TRF1 is that of a negative regulator of telomere length. Here we report another crucial function of TRF1 in telomeres. In conditional mouse TRF1 null mutant embryonic stem cells, TRF1 deletion induced growth defect and chromosomal instability. Although no clear telomere shortening or elongation was observed in short term cultured TRF1-deficient cells, abnormal telomere signals were observed, and TRF1-interacting telomere-binding factor, TIN2, lost telomeric association. Furthermore, another double-stranded telomeric DNA-binding factor, TRF2, also showed decreased telomeric association. Importantly, end-to-end fusions with detectable telomere signals at fusion points accumulated in TRF1-deficient cells. These results strongly suggest that TRF1 interacts with other telomere-binding molecules and integrates into the functional telomere structure.
- リンク情報
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- DOI
- https://doi.org/10.1074/jbc.M309138200
- CiNii Articles
- http://ci.nii.ac.jp/naid/80016428194
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/14559908
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000187722800074&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1074/jbc.M309138200
- ISSN : 0021-9258
- CiNii Articles ID : 80016428194
- PubMed ID : 14559908
- Web of Science ID : WOS:000187722800074