2018年1月1日
Cytoplasmic removal, enucleation, and cell fusion of mouse oocytes
Methods in Cell Biology
- ,
- ,
- 巻
- 144
- 号
- 開始ページ
- 459
- 終了ページ
- 474
- 記述言語
- 英語
- 掲載種別
- 論文集(書籍)内論文
- DOI
- 10.1016/bs.mcb.2018.03.038
- 出版者・発行元
- Academic Press Inc.
Meiotic divisions in females occur in fully grown oocytes that have a large cytoplasmic volume. The intracellular processes that are needed to accomplish meiotic divisions, such as spindle formation, chromosome segregation, and polar body extrusion, are controlled by the concerted actions of nuclear and cytoplasmic factors, which exhibit dynamic quantitative and spatiotemporal changes during meiotic maturation. Thus, distinguishing between meiotic controls that are mediated by cytoplasmic factors and those mediated by nuclear factors helps in the understanding of the mechanisms underlying meiotic divisions. Here, we describe a method to artificially modify the number of nuclei and the volume of the cytoplasm of mouse oocytes through cytoplasmic removal, enucleation, and cell fusion. The oocytes generated by this method are viable and undergo reproducible meiotic divisions exhibiting the effects of altered amounts of cytoplasmic and nuclear factors, which can be analyzed by various assays, such as live imaging microscopy.
- リンク情報
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- DOI
- https://doi.org/10.1016/bs.mcb.2018.03.038
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/29804683
- URL
- http://orcid.org/0000-0002-6486-7143
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85046618902&origin=inward
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85046618902&origin=inward
- ID情報
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- DOI : 10.1016/bs.mcb.2018.03.038
- ISSN : 0091-679X
- ORCIDのPut Code : 46947910
- PubMed ID : 29804683
- SCOPUS ID : 85046618902
- ORCIDで取得されたその他外部ID : a:1:{i:0;a:1:{s:4:"isbn";s:13:"9780128141441";}}