論文

査読有り 筆頭著者 国際誌
2007年11月

Contextual learning induces an increase in the number of hippocampal CA1 neurons expressing high levels of BDNF.

Neurobiology of learning and memory
  • Jenru Chen
  • ,
  • Takuma Kitanishi
  • ,
  • Takamitsu Ikeda
  • ,
  • Norio Matsuki
  • ,
  • Maki K Yamada

88
4
開始ページ
409
終了ページ
15
記述言語
英語
掲載種別

We examined behaviorally induced expression of brain-derived neurotrophic factor (BDNF) in area CA1 of the hippocampus. Sprague-Dawley rats were trained in a contextual fear conditioning (CFC) task, sacrificed 4h later, and their brains were processed for immunohistochemistry. We found distinctively high levels of BDNF immunoreactivity in a small number ( approximately 1%) of CA1 neurons in untrained animals. The number of these exceptional neurons, which are identified as BDNF(++) in this study, was increased by up to approximately 3% after CFC. This increase was blocked in the presence of a memory-impairing dose of a NMDA receptor antagonist (MK801 0.3 mg/kg, i.p.) given 30 min prior to training. The BDNF signal intensity in BDNF(++) neurons correlated with that of surrounding glutamic acid decarboxylase (GAD) 65. This correlation between GAD65 and BDNF signal intensities suggests that BDNF upregulation was associated with increased signaling via inhibitory GABAergic synapses that would lessen further intervening neuronal activity. Our observation that neurons which upregulate BDNF expression following a learning experience are rich in GAD65-enriched afferent synapses suggests that these neurons may have distinct roles in memory consolidation.

リンク情報
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/17765576
ID情報
  • ISSN : 1074-7427
  • PubMed ID : 17765576

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