論文

査読有り
2008年

Recombinant Sendai virus-mediated gene transfer to adipose tissue-derived stem cells (ASCs)

CELL TRANSPLANTATION
  • Hiroshi Yukawa
  • ,
  • Hirofumi Noguchi
  • ,
  • Koichi Oishi
  • ,
  • Takamichi Miyazaki
  • ,
  • Yasuo Kitagawa
  • ,
  • Makoto Inoue
  • ,
  • Mamoru Hasegawa
  • ,
  • Shuji Hayashi

17
1-2
開始ページ
43
終了ページ
50
記述言語
英語
掲載種別
研究論文(学術雑誌)
出版者・発行元
COGNIZANT COMMUNICATION CORP

Adipose tissue-derived stem cells (ASCs) are expected to have clinical applications as well as other stem cells, because ASCs can be obtained safely from adult donors and used in autologous therapies without concern about rejection and the need for immunosuppression. However, the use of gene transfer with Sendai virus (SeV) vectors, which can efficiently introduce foreign genes without toxicity into several cells, with ASCs has not yet been investigated. This study documents on the use of SeV vectors for gene transfer to ASCs. The dose-dependent GFP expression of ASCs transfected with SeV vectors after 48 h of culture at 37 degrees C was first evaluated. Next, the cellular toxicity of ASCs transfected with SeV vectors was verified. In addition, SeV vectors were compared with adenovirus (AdV) vectors. Finally, the time-dependent GFP expression of ASCs transfected with SeV vectors was evaluated. The results showed that transfection of ASCs with SeV vectors results in more efficient expression of transgene (GFP expression) in the ASCs than with AdV vectors after 48 h of culture at 37 degrees C. Moreover, while the transfection of ASCs with AdV vectors at high MOIs was cytotoxic (a lot of transfected cells died) that of ASCs with SeV vectors at high MOIs was not necessarily cytotoxic. In addition, the preservation of multilineage ASCs transfected with SeV was observed. In conclusion, this is the first report describing the successful use of SeV-mediated gene transfer in ASCs, and the results indicate that SeV may thus provide advantages with respect to safety issues in gene therapy.

リンク情報
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/18468234
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000254942200007&DestApp=WOS_CPL
ID情報
  • ISSN : 0963-6897
  • PubMed ID : 18468234
  • Web of Science ID : WOS:000254942200007

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