論文

査読有り 国際誌
2017年12月1日

Principles for the regulation of multiple developmental pathways by a versatile transcriptional factor, BLIMP1.

Nucleic acids research
  • Tadahiro Mitani
  • ,
  • Yukihiro Yabuta
  • ,
  • Hiroshi Ohta
  • ,
  • Tomonori Nakamura
  • ,
  • Chika Yamashiro
  • ,
  • Takuya Yamamoto
  • ,
  • Mitinori Saitou
  • ,
  • Kazuki Kurimoto

45
21
開始ページ
12152
終了ページ
12169
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/nar/gkx798

Single transcription factors (TFs) regulate multiple developmental pathways, but the underlying mechanisms remain unclear. Here, we quantitatively characterized the genome-wide occupancy profiles of BLIMP1, a key transcriptional regulator for diverse developmental processes, during the development of three germ-layer derivatives (photoreceptor precursors, embryonic intestinal epithelium and plasmablasts) and the germ cell lineage (primordial germ cells). We identified BLIMP1-binding sites shared among multiple developmental processes, and such sites were highly occupied by BLIMP1 with a stringent recognition motif and were located predominantly in promoter proximities. A subset of bindings common to all the lineages exhibited a new, strong recognition sequence, a GGGAAA repeat. Paradoxically, however, the shared/common bindings had only a slight impact on the associated gene expression. In contrast, BLIMP1 occupied more distal sites in a cell type-specific manner; despite lower occupancy and flexible sequence recognitions, such bindings contributed effectively to the repression of the associated genes. Recognition motifs of other key TFs in BLIMP1-binding sites had little impact on the expression-level changes. These findings suggest that the shared/common sites might serve as potential reservoirs of BLIMP1 that functions at the specific sites, providing the foundation for a unified understanding of the genome regulation by BLIMP1, and, possibly, TFs in general.

リンク情報
DOI
https://doi.org/10.1093/nar/gkx798
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28981894
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5716175
URL
http://europepmc.org/abstract/med/28981894
ID情報
  • DOI : 10.1093/nar/gkx798
  • ORCIDのPut Code : 37563340
  • PubMed ID : 28981894
  • PubMed Central 記事ID : PMC5716175

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