論文

査読有り
2017年3月

Discrimination of Stem Cell Status after Subjecting Cynomolgus Monkey Pluripotent Stem Cells to Naive Conversion

SCIENTIFIC REPORTS
  • Arata Honda
  • ,
  • Yoshihiro Kawano
  • ,
  • Haruna Izu
  • ,
  • Narantsog Choijookhuu
  • ,
  • Kimiko Honsho
  • ,
  • Tomonori Nakamura
  • ,
  • Yukihiro Yabuta
  • ,
  • Takuya Yamamoto
  • ,
  • Yasuhiro Takashima
  • ,
  • Michiko Hirose
  • ,
  • Tadashi Sankai
  • ,
  • Yoshitaka Hishikawa
  • ,
  • Atsuo Ogura
  • ,
  • Mitinori Saitou

7
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/srep45285
出版者・発行元
NATURE PUBLISHING GROUP

Experimental animal models have played an indispensable role in the development of human induced pluripotent stem cell (iPSC) research. The derivation of high-quality (so-called "true naive state") iPSCs of non-human primates enhances their application and safety for human regenerative medicine. Although several attempts have been made to convert human and non-human primate PSCs into a truly naive state, it is unclear which evaluation methods can discriminate them as being truly naive. Here we attempted to derive naive cynomolgus monkey (Cm) (Macaca fascicularis) embryonic stem cells (ESCs) and iPSCs. Several characteristics of naive Cm ESCs including colony morphology, appearance of naive-related mRNAs and proteins, leukaemia inhibitory factor dependency, and mitochondrial respiration were confirmed. Next, we generated Cm iPSCs and converted them to a naive state. Transcriptomic comparison of PSCs with early Cm embryos elucidated the partial achievement (termed naive-like) of their conversion. When these were subjected to in vitro neural differentiation, enhanced differentiating capacities were observed after naive-like conversion, but some lines exhibited heterogeneity. The difficulty of achieving contribution to chimeric mouse embryos was also demonstrated. These results suggest that Cm PSCs could ameliorate their in vitro neural differentiation potential even though they could not display true naive characteristics.

Web of Science ® 被引用回数 : 11

リンク情報
DOI
https://doi.org/10.1038/srep45285
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28349944
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5368663
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000397535800001&DestApp=WOS_CPL
ID情報
  • DOI : 10.1038/srep45285
  • ISSN : 2045-2322
  • ORCIDのPut Code : 31841208
  • PubMed ID : 28349944
  • PubMed Central 記事ID : PMC5368663
  • Web of Science ID : WOS:000397535800001

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