論文

査読有り
2016年9月

A developmental coordinate of pluripotency among mice, monkeys and humans

NATURE
  • Tomonori Nakamura
  • ,
  • Ikuhiro Okamoto
  • ,
  • Kotaro Sasaki
  • ,
  • Yukihiro Yabuta
  • ,
  • Chizuru Iwatani
  • ,
  • Hideaki Tsuchiya
  • ,
  • Yasunari Seita
  • ,
  • Shinichiro Nakamura
  • ,
  • Takuya Yamamoto
  • ,
  • Mitinori Saitou

537
7618
開始ページ
57
終了ページ
62
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/nature19096
出版者・発行元
NATURE PUBLISHING GROUP

The epiblast (EPI) is the origin of all somatic and germ cells in mammals, and of pluripotent stem cells in vitro. To explore the ontogeny of human and primate pluripotency, here we perform comprehensive single-cell RNA sequencing for pre and post-implantation EPI development in cynomolgus monkeys (Macacafascicularis). We show that after specification in the blastocysts, EPI from cynomolgus monkeys (cyEPI) undergoes major transcriptome changes on implantation. Thereafter, while generating gastrulating cells, cyEPI stably maintains its transcriptome over a week, retains a unique set of pluripotency genes and acquires properties for 'neuron differentiation'. Human and monkey pluripotent stem cells show the highest similarity to post-implantation late cyEPI, which, despite co-existing with gastrulating cells, bears characteristics of pre-gastrulating mouse EPI and epiblast-like cells in vitro. These findings not only reveal the divergence and coherence of EPI development, but also identify a developmental coordinate of the spectrum of pluripotency among key species, providing a basis for better regulation of human pluripotency in vitro.

Web of Science ® 被引用回数 : 222

リンク情報
DOI
https://doi.org/10.1038/nature19096
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27556940
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000382426900037&DestApp=WOS_CPL
ID情報
  • DOI : 10.1038/nature19096
  • ISSN : 0028-0836
  • eISSN : 1476-4687
  • ORCIDのPut Code : 27321247
  • PubMed ID : 27556940
  • Web of Science ID : WOS:000382426900037

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