論文

査読有り 国際誌
2021年4月5日

Capturing human trophoblast development with naive pluripotent stem cells in vitro.

Cell stem cell
  • Shingo Io
  • ,
  • Mio Kabata
  • ,
  • Yoshiki Iemura
  • ,
  • Katsunori Semi
  • ,
  • Nobuhiro Morone
  • ,
  • Atsutaka Minagawa
  • ,
  • Bo Wang
  • ,
  • Ikuhiro Okamoto
  • ,
  • Tomonori Nakamura
  • ,
  • Yoji Kojima
  • ,
  • Chizuru Iwatani
  • ,
  • Hideaki Tsuchiya
  • ,
  • Belinda Kaswandy
  • ,
  • Eiji Kondoh
  • ,
  • Shin Kaneko
  • ,
  • Knut Woltjen
  • ,
  • Mitinori Saitou
  • ,
  • Takuya Yamamoto
  • ,
  • Masaki Mandai
  • ,
  • Yasuhiro Takashima

記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.stem.2021.03.013

Trophoblasts are extraembryonic cells that are essential for maintaining pregnancy. Human trophoblasts arise from the morula as trophectoderm (TE), which, after implantation, differentiates into cytotrophoblasts (CTs), syncytiotrophoblasts (STs), and extravillous trophoblasts (EVTs), composing the placenta. Here we show that naïve, but not primed, human pluripotent stem cells (PSCs) recapitulate trophoblast development. Naive PSC-derived TE and CTs (nCTs) recreated human and monkey TE-to-CT transition. nCTs self-renewed as CT stem cells and had the characteristics of proliferating villous CTs and CTs in the cell column of the first trimester. Notably, although primed PSCs differentiated into trophoblast-like cells (BMP4, A83-01, and PD173074 [BAP]-treated primed PSCs [pBAPs]), pBAPs were distinct from nCTs and human placenta-derived CT stem cells, exhibiting properties consistent with the amnion. Our findings establish an authentic paradigm for human trophoblast development, demonstrating the invaluable properties of naive human PSCs. Our system provides a platform to study the molecular mechanisms underlying trophoblast development and related diseases.

リンク情報
DOI
https://doi.org/10.1016/j.stem.2021.03.013
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/33831365
ID情報
  • DOI : 10.1016/j.stem.2021.03.013
  • PubMed ID : 33831365

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