2012年2月
Effect of dielectric spacer thickness on signal intensity of surface plasmon field-enhanced fluorescence spectroscopy
ANALYTICAL BIOCHEMISTRY
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- ,
- ,
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- 巻
- 421
- 号
- 2
- 開始ページ
- 632
- 終了ページ
- 639
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.ab.2011.12.008
- 出版者・発行元
- ACADEMIC PRESS INC ELSEVIER SCIENCE
Surface plasmon field-enhanced fluorescence spectroscopy (SPFS) combines enhanced field platform and fluorescence detection. Its advantages are the strong intensity of the electromagnetic field and the high signal/noise (S/N) ratio due to the localized evanescent field at the water/metal interface. However, the energy transfer from the fluorophore to the metal surface diminishes the fluorescence intensity, and this reduces the sensitivity. In this study, we tested whether polystyrene (PSt) could act as a dielectric layer to suppress the energy transfer from the fluorophore to the metal surface. We hypothesized that this would improve the sensitivity of SPFS-based immunoassays. We used alpha-fetoprotein (AFP) as a model tumor biomarker in the sandwich-type immunoassay. We determined the relationship between fluorescent signal intensity and PSt layer thickness and compared this to theoretical predictions. We found that the fluorescence signal increased by optimally controlling the thickness of the PSt layer. Our results indicated that the SPFS-based immunoassay is a promising clinical diagnostic tool for quantitatively determining the concentrations of low-level biomarkers in blood samples. (C) 2011 Elsevier Inc. All rights reserved.
- リンク情報
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- DOI
- https://doi.org/10.1016/j.ab.2011.12.008
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000300528400036&DestApp=WOS_CPL
- URL
- http://www.scopus.com/inward/record.url?eid=2-s2.0-84859713199&partnerID=MN8TOARS
- URL
- http://orcid.org/0000-0002-6683-4513
- ID情報
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- DOI : 10.1016/j.ab.2011.12.008
- ISSN : 0003-2697
- ORCIDのPut Code : 37221007
- SCOPUS ID : 84859713199
- Web of Science ID : WOS:000300528400036