論文

査読有り
2012年3月

Small variation of glucosinolate composition in Japanese cultivars of radish (Raphanus sativus L.) requires simple quantitative analysis for breeding of glucosinolate component

BREEDING SCIENCE
  • Masahiko Ishida
  • ,
  • Masayasu Nagata
  • ,
  • Takayoshi Ohara
  • ,
  • Tomohiro Kakizaki
  • ,
  • Katunori Hatakeyama
  • ,
  • Takeshi Nishio

62
1
開始ページ
63
終了ページ
70
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1270/jsbbs.62.63
出版者・発行元
JAPANESE SOC BREEDING

To reveal varietally differing glucosinolate (GSL) contents in radish (Raphanus sativus L.) cultivated in Japan, the total and individual GSLs of 28 cultivars were analyzed using high-performance liquid chromatography. In these cultivars, GSL types including three aliphatic GSLs (glucoraphenin, glucoerucin, and 4-methylthio-3-butenyl GSL (4MTB-GSL)) and three indolyl GSLs (4-hydroxy-glucobrassicin, glucobrassicin, and 4-methoxy-glucobrassicin) were detected. No cultivar-specific type of GSL was identified. The dominant GSL was 4MTB-GSL, but its contents differed remarkably: 8.6 mu mol/g in 'Koushin' to 135.7 mu mol/g in 'Karami 199'. Over about 90% of all GSLs in Japanese radish type are 4MTB-GSL, a higher percentage than in Chinese or European garden radish cultivars. A simple, rapid method for estimating total GSL contents in crude extracts was established because of the small variation of glucosinolate composition in Japanese cultivars. The total GSL content can be estimated using an equation for prediction with absorbance at 425 nm in a mixture of GSL crude extract and palladium (II) chloride solution: Total GSL (mu mol/g) = 305.47 x A(425) - 29.66. Its coefficient of determination (R-2) and standard error of prediction (SEP) are 0.968 and 8.052. This method enables total GSL content estimation from more than 200 samples per person per day.

Web of Science ® 被引用回数 : 24

リンク情報
DOI
https://doi.org/10.1270/jsbbs.62.63
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23136515
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000304511200009&DestApp=WOS_CPL
ID情報
  • DOI : 10.1270/jsbbs.62.63
  • ISSN : 1344-7610
  • eISSN : 1347-3735
  • PubMed ID : 23136515
  • Web of Science ID : WOS:000304511200009

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