論文

査読有り
2017年4月

The tandem repeated organization of NB-LRR genes in the clubroot-resistant CRb locus in Brassica rapa L.

MOLECULAR GENETICS AND GENOMICS
  • Katsunori Hatakeyama
  • ,
  • Tomohisa Niwa
  • ,
  • Takeyuki Kato
  • ,
  • Takayoshi Ohara
  • ,
  • Tomohiro Kakizaki
  • ,
  • Satoru Matsumoto

292
2
開始ページ
397
終了ページ
405
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00438-016-1281-1
出版者・発行元
SPRINGER HEIDELBERG

To facilitate prevention of clubroot disease, a major threat to the successful cultivation of Chinese cabbage (Brassica rapa L.), we bred clubroot-resistant (CR) cultivars by introducing resistance genes from CR turnips via conventional breeding. Among 11 CR loci found in B. rapa, we identified CRb in Chinese cabbage cultivar 'CR Shinki' as a single dominant gene for resistance against Plasmodiophora brassicae pathotype group 3, against which the stacking of Crr1 and Crr2 loci was not effective. However, the precise location and pathotype specificity of CRb have been controversial, because CRa and Rcr1 also map near this locus. Previously, our fine-mapping study revealed that CRb is located in a 140-kb genomic region on chromosome A03. Here, we determined the nucleotide sequence of an approximately 64-kb candidate region in the resistant line; this region contains six open reading frames (ORFs) similar to NB-LRR encoding genes that are predicted to occur in tandem with the same orientation. Among the six ORFs present, only four on the genome of the resistant line showed a strong DNA sequence identity with each other, and only one of those four could confer resistance to P. brassicae isolate No. 14 of the pathotype group 3. These results suggest that these genes evolved through recent gene duplication and uneven crossover events that could lead to the acquisition of clubroot resistance. The DNA sequence of the functional ORF was identical to that of the previously cloned CRa gene; thus, we showed that the independently identified CRb and CRa are one and the same clubroot-resistance gene.

Web of Science ® 被引用回数 : 26

リンク情報
DOI
https://doi.org/10.1007/s00438-016-1281-1
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28013378
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000398590000011&DestApp=WOS_CPL
ID情報
  • DOI : 10.1007/s00438-016-1281-1
  • ISSN : 1617-4615
  • eISSN : 1617-4623
  • PubMed ID : 28013378
  • Web of Science ID : WOS:000398590000011

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