論文

査読有り
2011年6月

Development of a simple and rapid extraction method of glucosinolates from radish roots

BREEDING SCIENCE
  • Masahiko Ishida
  • ,
  • Tomohiro Kakizaki
  • ,
  • Takayoshi Ohara
  • ,
  • Yasujiro Morimitsu

61
2
開始ページ
208
終了ページ
211
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1270/jsbbs.61.208
出版者・発行元
JAPANESE SOC BREEDING

A simple and rapid method for the extraction of crude glucosinolates from powder of freeze-dried radish roots was developed using a common solvent, methanol/water=80 : 20 (v/v) at room temperature. Hot methanol is not required for the extraction procedure of this new method. Both 4-methylthio-3-butenyl glucosinolate, which is a major glucosinolate in radish root, and total glucosinolates of Japanese radish cv. 'Karami 199' were quantified using HPLC. The amounts of glucosinolate extracted using this new method were 4 6% higher than those obtained using the conventional method with hot methanol. To compare the new method with the conventional method, the amounts of glucosinolate extracted from roots of eight cultivars in Japanese radish and five breeding lines were estimated. A high positive correlation was found between them. This simple and rapid method 1) obviates hot methanol for glucosinolate extraction and 2) requires only one-third of the extraction time of the conventional method, enabling glucosinolate extraction from about 150 samples by one person in a single day.

Web of Science ® 被引用回数 : 16

リンク情報
DOI
https://doi.org/10.1270/jsbbs.61.208
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000293495200015&DestApp=WOS_CPL
ID情報
  • DOI : 10.1270/jsbbs.61.208
  • ISSN : 1344-7610
  • eISSN : 1347-3735
  • Web of Science ID : WOS:000293495200015

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