論文

国際誌
2022年3月

Synthesis of Crosslinked 2′‐OMe RNA Duplexes Using 2‐Amino‐6‐Vinylpurine and Their Application for Effective Inhibition of miRNA Function

Current Protocols
  • Ahmed Mostafa Abdelhady
  • ,
  • Yu Hirano
  • ,
  • Kazumitsu Onizuka
  • ,
  • Hidenori Okamura
  • ,
  • Yasuo Komatsu
  • ,
  • Fumi Nagatsugi

2
3
開始ページ
e386
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/cpz1.386
出版者・発行元
Wiley

Crosslinking reactions to nucleic acids are an effective way to prepare stable complexes formed by covalent bonding. We demonstrated that fully 2'-O-methylated (2'-OMe) RNAs having a 2-amino-6-vinylpurine (AVP) exhibited an efficient crosslinking to uracil in the target RNA. Recently, we reported the preparation of crosslinked 2'-OMe RNA duplexes using AVP and the anti-miRNA oligonucleotides (AMOs) containing crosslinked duplexes at the terminal positions. These AMOs exhibited efficient microRNA (miRNA) inhibition at very low concentrations. In this article, we describe the chemical synthesis of 2'-OMe oligonucleotides containing AVP and preparation of the AMOs bearing crosslinked 2'-OMe RNA duplexes using AVP. In addition, we describe in detail the miRNA inhibition assay using these AMOs. © 2022 Wiley Periodicals LLC. Basic Protocol 1: Synthesis of phosphoramidite of 2-amino-6-vinylguanosine derivative Basic Protocol 2: Synthesis of AVP-2'-OMe RNA Basic Protocol 3: Evaluation of the crosslink reactivity of CFO containing AVP to the 2'-OMe RNA and preparation of AMOs containing crosslinked duplex Basic Protocol 4: miRNA inhibition assays.

リンク情報
DOI
https://doi.org/10.1002/cpz1.386
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/35316581
URL
https://onlinelibrary.wiley.com/doi/pdf/10.1002/cpz1.386
URL
https://onlinelibrary.wiley.com/doi/full-xml/10.1002/cpz1.386
ID情報
  • DOI : 10.1002/cpz1.386
  • ISSN : 2691-1299
  • eISSN : 2691-1299
  • PubMed ID : 35316581

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