論文

国際誌
2020年9月30日

Simultaneous TALEN-mediated knockout of chrysanthemum DMC1 genes confers male and female sterility.

Scientific reports
  • Harue Shinoyama
  • ,
  • Hiroaki Ichikawa
  • ,
  • Ayako Nishizawa-Yokoi
  • ,
  • Mikhail Skaptsov
  • ,
  • Seiichi Toki

10
1
開始ページ
16165
終了ページ
16165
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41598-020-72356-1

Genome editing has become one of the key technologies for plant breeding. However, in polyploid species such as chrysanthemum, knockout of all loci of multiple genes is needed to eliminate functional redundancies. We identified six cDNAs for the CmDMC1 genes involved in meiotic homologous recombination in chrysanthemum. Since all six cDNAs harbored a homologous core region, simultaneous knockout via TALEN-mediated genome editing should be possible. We isolated the CmDMC1 loci corresponding to the six cDNAs and constructed a TALEN-expression vector bearing a CmDMC1 target site containing the homologous core region. After transforming two chrysanthemum cultivars with the TALEN-expression vector, seven lines exhibited disruption of all six CmDMC1 loci at the target site as well as stable male and female sterility at 10-30 °C. This strategy to produce completely sterile plants could be widely applicable to prevent the risk of transgene flow from transgenic plants to their wild relatives.

リンク情報
DOI
https://doi.org/10.1038/s41598-020-72356-1
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32999297
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7527520
ID情報
  • DOI : 10.1038/s41598-020-72356-1
  • PubMed ID : 32999297
  • PubMed Central 記事ID : PMC7527520

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