Hiroshi Tokumitsu

Profile Information

Affiliation: Professor, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University

Degree: Ph.D.(Mar, 1991, Nagoya Univ.)

Researcher number: 20237077
J-GLOBAL ID: 200901005162981897
researchmap Member ID: 5000079512

External link: http://www.okayama-u.ac.jp/user/saibou/

Research Interests

Research Areas

Research History

2021 - Present

教授, 学術研究院ヘルスシステム統合科学学域, 岡山大学
2018 - Present

Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
2012 - 2018

Graduate School of Natural Science and Technology, Okayama University
2007 - 2012

Faculty of Medicine, Kagawa University
2003 - 2007

Faculty of Medicine, Kagawa University
1999 - 2003

Faculty of Medicine, Kagawa Medical University
1996 - 1999

主任研究員, （株）ヘリックス研究所
1993 - 1996

博士研究員, オレゴン健康科学大学 Vollum 研究所
1991 - 1994

School of Medicine, Nagoya University
1992 - 1993

博士研究員, DNAX 分子細胞生物学研究所
1991 - 1991

School of Medicine, Nagoya University

Papers

113

Development of a novel AAK1 inhibitor via Kinobeads-based screening.

Akari Yoshida, Satomi Ohtsuka, Fumiya Matsumoto, Tomoyuki Miyagawa, Rei Okino, Yumeya Ikeda, Natsume Tada, Akira Gotoh, Masaki Magari, Naoya Hatano …

Scientific reports, 14(1) 6723-6723, Mar 20, 2024 Peer-reviewedLast authorCorresponding author
Transcriptional, biochemical, and immunohistochemical analyses of CaMKKβ/2 splice variants that co-localize with CaMKIV in spermatids.

Satomi Ohtsuka, Yumi Miyai, Hiroyuki Mima, Masaki Magari, Yoichi Chiba, Futoshi Suizu, Hiroyuki Sakagami, Masaki Ueno, Hiroshi Tokumitsu

Cell calcium, 117 102820-102820, Jan, 2024 Peer-reviewedLast authorCorresponding author
PHI-1, an Endogenous Inhibitor Protein for Protein Phosphatase-1 and a Pan-Cancer Marker, Regulates Raf-1 Proteostasis

Jason A. Kirkbride, Garbo Young Nilsson, Jee In Kim, Kosuke Takeya, Yoshinori Tanaka, Hiroshi Tokumitsu, Futoshi Suizu, Masumi Eto

Biomolecules, 13(12) 1741-1741, Dec 4, 2023 Peer-reviewed
Rapid detection of calmodulin/target interaction via the proximity biotinylation method.

Kento Nlandu Nakamura, Haruki Yamauchi, Hiroyuki Mima, Yerun Chen, Satomi Ohtsuka, Masaki Magari, Ryo Morishita, Hiroshi Tokumitsu

Biochemical and biophysical research communications, 659 29-33, Apr 1, 2023 Peer-reviewedLast authorCorresponding author
Substrate recognition by Arg/Pro-rich insert domain in calcium/calmodulin-dependent protein kinase kinase for target protein kinases.

Riku Kaneshige, Satomi Ohtsuka, Yuhei Harada, Issei Kawamata, Masaki Magari, Naoki Kanayama, Naoya Hatano, Hiroyuki Sakagami, Hiroshi Tokumitsu

The FEBS journal, 289(19) 5971-5984, Oct, 2022 Peer-reviewedLast authorCorresponding author
The immunoreceptor SLAMF8 promotes the differentiation of follicular dendritic cell‐dependent monocytic cells with B cell‐activating ability

Masaki Magari, Miku Nishioka, Tomomi Hari, Sayaka Ogawa, Kaho Takahashi, Naoya Hatano, Naoki Kanayama, Junichiro Futami, Hiroshi Tokumitsu

FEBS Letters, 596(20) 2659-2667, Oct, 2022 Peer-reviewedLast author
Molecular Mechanisms Underlying Ca2+/Calmodulin-Dependent Protein Kinase Kinase Signal Transduction

Hiroshi Tokumitsu, Hiroyuki Sakagami

International Journal of Molecular Sciences, 23(19) 11025-11025, Sep 20, 2022 Peer-reviewedLead authorCorresponding author
Conformation-Dependent Reversible Interaction of Ca2+/Calmodulin-Dependent Protein Kinase Kinase with an Inhibitor, TIM-063.

Satomi Ohtsuka, Taisei Okumura, Yuna Τabuchi, Tomoyuki Miyagawa, Naoki Kanayama, Masaki Magari, Naoya Hatano, Hiroyuki Sakagami, Futoshi Suizu, Teruhiko Ishikawa …

Biochemistry, 61(7) 545-553, Apr 5, 2022 Peer-reviewedLast authorCorresponding author
Oligomerization of Ca²⁺/calmodulin-dependent protein kinase kinase

Yusei Fukumoto, Yuhei Harada, Satomi Ohtsuka, Naoki Kanayama, Masaki Magari, Naoya Hatano, Hiroyuki Sakagami, Hiroshi Tokumitsu

Biochemical and Biophysical Research Communications, 587 160-165, Jan, 2022 Peer-reviewedLast authorCorresponding author
A temporal Ca²⁺-desensitization of myosin light chain kinase in phasic smooth muscles induced by CaMKKβ/PP2A pathways.

Toshio Kitazawa, Toshiyasu Matsui, Shuichi Katsuki, Akira Goto, Kai Akagi, Naoya Hatano, Hiroshi Tokumitsu, Kosuke Takeya, Masumi Eto

American Journal of Physiology-Cell Physiology, 321(3) C549-C558, Sep, 2021 Peer-reviewed
Regulation of the tubulin polymerization-promoting protein by Ca²⁺/S100 proteins

Seita Doi, Naoki Fujioka, Satomi Ohtsuka, Rina Kondo, Maho Yamamoto, Miwako Denda, Masaki Magari, Naoki Kanayama, Naoya Hatano, Ryo Morishita …

Cell Calcium, 96 102404, Jul, 2021 Peer-reviewedLast authorCorresponding author
Identification and Biochemical Characterization of High Mobility Group Protein 20A as a Novel Ca²⁺/S100A6 Target

Maho Yamamoto, Rina Kondo, Haruka Hozumi, Seita Doi, Miwako Denda, Masaki Magari, Naoki Kanayama, Naoya Hatano, Ryo Morishita, Hiroshi Tokumitsu

Biomolecules, 11(4) 510, Mar, 2021 Peer-reviewedLast authorCorresponding author
Development and Characterization of Novel Molecular Probes for Ca²⁺/Calmodulin-Dependent Protein Kinase Kinase, Derived from STO-609.

Satomi Ohtsuka, Yui Ozeki, Moeno Fujiwara, Tomoyuki Miyagawa, Naoki Kanayama, Masaki Magari, Naoya Hatano, Futoshi Suizu, Teruhiko Ishikawa, Hiroshi Tokumitsu

Biochemistry, 59(17) 1701-1710, May 5, 2020 Peer-reviewedLast authorCorresponding author
Phosphorylation and dephosphorylation of Ca²⁺/calmodulin-dependent protein kinase kinase β at Thr144 in HeLa cells.

Shota Takabatake, Yusei Fukumoto, Satomi Ohtsuka, Naoki Kanayama, Masaki Magari, Hiroyuki Sakagami, Naoya Hatano, Hiroshi Tokumitsu

Biochemical and Biophysical Research Communications, 525(1) 251-257, Feb 19, 2020 Peer-reviewedLast authorCorresponding author
IL-34 cell surface localization regulated by the 78 kDa glucose-regulated protein facilitates the differentiation of monocytic cells.

Sayaka Ogawa, Yukiko Matsuoka, Miho Takada, Kazue Matsui, Fumihiro Yamane, Eri Kubota, Shiori Yasuhara, Kentaro Hieda, Naoki Kanayama, Naoya Hatano …

The Journal of Biological Chemistry, 294(7) 2386-2396, Feb, 2019 Peer-reviewed
Regulation of Ca²⁺/calmodulin-dependent protein kinase kinase β by cAMP signaling.

Shota Takabatake, Satomi Ohtsuka, Takeyuki Sugawara T, Naoya Hatano, Naoki Kanayama, Masaki Magari, Hiroyuki Sakagami, Hiroshi Tokumitsu

iochimica et Biophysica Acta. General Subjects, 1863(4) 672-680, Jan, 2019 Peer-reviewedLast authorCorresponding author
Interaction of S100A6 with Target Proteins In Vitro and in Living Cells.

Kyohei Sakane, Fuminori Yamaguchi, Mitsumasa Tsuchiya, Rina Kondo, Naoki Kanayama, Masaki Magari, Naoya Hatano, Ryoji Kobayashi, Hiroshi Tokumitsu

Methods in Molecular Biology (Clifton, N.J.), 1929 367-377, 2019 Peer-reviewedLast authorCorresponding author
ヘルスシステムの理解とその応用濾胞樹状細胞による抗体の親和性成熟の制御機構の解明(Interdisciplinary Science and Engineering in Health Systems Immunological functions of follicular dendritic cells on affinity maturation of antibody)

Magari Masaki, Ogawa Sayaka, Matsuoka Yukiko, Takada Miho, Kanayama Naoki, Tokumitsu Hiroshi

生物物理, 58(Suppl.1-2) S197-S197, Aug, 2018
Expression of Collagenase is Regulated by the VarS/VarA Two-Component Regulatory System in Vibrio alginolyticus.

Takehiko Mima, Kazuyoshi Gotoh, Yumiko Yamamoto, Keiko Maeda, Taku Shirakawa, Shunsuke Matsui, Yumi Murata, Takaki Koide, Hiroshi Tokumitsu, Osamu Matsushita

The Journal of Membrane Biology, 251(1) 51-63, Feb, 2018 Peer-reviewed
AMP-activated protein kinase-mediated feedback phosphorylation controls the Ca2+/calmodulin (CaM) dependence of Ca2+/CaM-dependent protein kinase kinase

Akihiro Nakanishi, Naoya Hatano, Yuya Fujiwara, Arian Sha'ri, Shota Takabatake, Hiroki Akano, Naoki Kanayama, Masaki Magari, Naohito Nozaki, Hiroshi Tokumitsu

JOURNAL OF BIOLOGICAL CHEMISTRY, 292(48) 19804-19813, Dec, 2017 Peer-reviewedLast authorCorresponding author

Misc.

CaMKKの基質認識機構の解明と特異的阻害分子開発への応用

川俣一晟, 美馬光志, 山内陽生, 北前勝哉, 山内香奈, 大塚里美, 曲正樹, 金山直樹, 徳光浩

日本生化学会大会(Web), 95th, 2022
CaMKK阻害剤(TIM-063)を用いた阻害剤プロテオミクス解析

大塚里美, 波多野直哉, 奥村太晟, 澤直樹, 田邊史子, 傳田美和子, 金山直樹, 曲正樹, 森下了, 石川彰彦 …

日本生化学会大会(Web), 94th, 2021
筋肉特異的Calmodulin結合分子,Striated Muscle Activator of Rho Signaling(STARS)の分子間相互作用解析

赤木魁, 田中啓之, 金山直樹, 曲正樹, 波多野直哉, 徳光浩

日本分子生物学会年会プログラム・要旨集(Web), 42nd, 2019
CaMKKβのリン酸化/脱リン酸化による動的制御機構の解明

高畠翔太, 福本侑世, 金山直樹, 曲正樹, 波多野直哉, 徳光浩

日本分子生物学会年会プログラム・要旨集(Web), 42nd, 2019
新規S100A6標的分子(HMG20A)の同定と相互作用解析

山本真穂, 近藤里奈, 傳田美和子, 土居青太, 金山直樹, 曲正樹, 森下了, 徳光浩

日本生化学会大会(Web), 92nd, 2019
Ca²⁺/calmodulin-dependent protein kinase kinase—カルシウムシグナル伝達から創薬へ—

Hiroshi Tokumitsu

Journal of Japanese Biochemical Society, 90(4) 452-461, Aug, 2018 Peer-reviewed
インターラクトーム解析法を用いたヒトS100A6標的分子の探索

坂根恭平, 西口みゆ, 古谷雄穂, 傳田美和子, 山口文徳, 曲正樹, 金山直樹, 森下了, 徳光浩

日本生化学会大会(Web), 88th 1P0204 (WEB ONLY)-[1P0204], Dec, 2015
Ca²⁺/Calmodulin結合型転写因子の網羅的同定

太尾田泰成, 大西和貴, 古谷雄穂, 傳田美和子, 金山直樹, 曲正樹, 森下了, 徳光浩

日本生化学会大会(Web), 88th 3P0185 (WEB ONLY)-[3P0185], 2015
ヒトCalmodulin標的分子の網羅的同定

古谷雄穂, 西口みゆ, 傳田美和子, 曲正樹, 金山直樹, 森下了, 徳光浩

日本生化学会大会(Web), 87th 4T14A-01(3P-324) (WEB ONLY)-01], Oct, 2014
S 100 PROTEINS MODULATE PROTEIN PHOSPHATASE 5 FUNCTION : A LINK BETWEEN Ca2+ SIGNAL TRANSDUCTION AND PROTEIN DEPHOSPHORYLATION

Fuminori Yamaguchi, Yoshinori Umeda, Seiko Shimamoto, Mitsumasa Tsuchiya, Hiroshi Tokumitsu, Masaaki Tokuda, Ryoji Kobayashi

JOURNAL OF PHYSIOLOGICAL SCIENCES, 63 S139-S139, 2013
[Small-molecule modulator of calcium signaling].

Kobayashi R, Tokumitsu H, Fujimoto T

Nihon rinsho. Japanese journal of clinical medicine, 70 Suppl 8 277-280, Nov, 2012 Peer-reviewed
ATPアナログを用いたCaMKKの新規標的基質分子の同定

藤本智仁, 波多野直哉, 野崎直仁, 横倉沙紀, 本, 小林良二, 徳光浩

日本生化学会大会プログラム・講演要旨集, 84回 3P-0376, Sep, 2011
CaMKKの自己リン酸化による活性調節メカニズム

徳光浩, 横倉沙紀, 本, 波多野直哉, 藤本智仁, 小林良二

日本生化学会大会プログラム・講演要旨集, 84回 3T10p-6, Sep, 2011
新しいニューロン性カルモジュリン結合蛋白PRG-1(可塑性関連遺伝子1)(PRG-1 (plasticity related gene 1), a novel neuronal calmodulin-binding protein)

徳光浩, 波多野直哉, 土屋光正, 揺本沙紀, 藤本智仁, 小原直樹, 小林良二, 阪上洋行

日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, 83回・33回 3P-0346, Dec, 2010
Calmodulin-Kinases: Modulators of Neuronal Development and Plasticity (vol 59, pg 914, 2008)

Gary A. Wayman, Yong-Seok Lee, Hiroshi Tokumitsu, Alcino J. Silva, Thomas R. Soderling

NEURON, 64(4) 590-590, Nov, 2009
Exendin-4 regulates glucokinase expression by CaMKK/CaMKIV pathway in pancreatic beta-cell line

K. Murao, J. Li, H. Imachi, T. Muraoka, H. Masugata, G. X. Zhang, R. Kobayashi, T. Ishida, H. Tokumitsu

DIABETES OBESITY & METABOLISM, 11(10) 939-946, Oct, 2009 Peer-reviewed
新規カルモデュリン結合タンパク質、PRG-1の同定と生化学的解析

土屋光正, 波多野直哉, 揺本沙紀, 倉, 小林良二, 阪上洋行, 徳光浩

日本生化学会大会プログラム・講演要旨集, 82回 4T4a-2, Sep, 2009
機能的プロテオミクス法を用いたカルモデュリン結合タンパク質の網羅的探索

揺本沙紀, 横倉, 藤本智仁, 波多野直哉, 土屋光正, 中野重治, 掛川寿夫, 小林良二, 徳光浩

日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, 81回・31回 1T15-12, Nov, 2008
Signal transduction of CaM-kinase cascade and its specific inhibitor

H Tokumitsu

JOURNAL OF PHARMACOLOGICAL SCIENCES, 94 38P-38P, 2004
Neuronal calcium sensor proteins are direct targets of the insulinotropic agent repaglinide

M Okada, D Takezawa, S Tachibanaki, S Kawamura, H Tokumitsu, R Kobayashi

BIOCHEMICAL JOURNAL, 375(1) 87-97, Oct, 2003

Books and Other Publications

Ca²⁺/calmodulin-dependent protein kinase kinase —カルシウムシグナル伝達から創薬へ—

徳光浩

生化学（公益財団法人日本生化学会）, Aug, 2018
Ca²⁺/カルモデュリン依存性蛋白質リン酸化酵素カスケ-ドと生理機能

徳光浩

蛋白質核酸酵素（共立出版）, Apr, 1998

Research Projects

CaMKKシグナル伝達の制御機構解明とそれに基づく分子標的薬創製

科学研究費助成事業基盤研究(B), 基盤研究(B), 日本学術振興会, Apr, 2021 - Mar, 2024

徳光浩, 石川彰彦, 渡辺泰男, 曲正樹
Regulatory mechanism of transcription by ciliary proteins.

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2019 - Mar, 2022

Suizu Futoshi
Regulation of CaMKKbeta/AMPK signaling and drug development

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2018 - Mar, 2021

Tokumitsu Hiroshi
Improvement of an animal cell-displaying technology by using a splicing factor

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2015 - Mar, 2019

Kanayama Naoki
Phosphorylation-dependent regulatory mechanism of calmoudlin-kinase cascade

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2014 - Mar, 2017

Tokumitsu Hiroshi, HATANO Naoya
Therapeutic strategy for lipotoxicity of pancreatic beta-cells

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2012 - Mar, 2015

MURAO Koji, IMACHI Hitomi, TOKUMITSU Hiroshi, OHMORI Koji
Comprehensive identification and signal transduction analysis of calmodulin-targets by functional proteomics

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, 2009 - 2011

TOKUMITSU Hiroshi, KOBAYASHI Ryouji, HATANO Naoya
Identification and characterization of a novel target for CaM-kinase cascade

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, 2007 - 2008

TOKUMITSU Hiroshi, KOBAYASHI Ryoji
Mechanisms of insulin gene expression and regeneration of pancreatic beta-cell

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, 2007 - 2008

ISHIDA Toshihiko, MURAO Koji, TOKUMITSU Hiroshi, IMACHI Hitomi
The development of the molecular target drugs for intracellular calcium signal pathway

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, 2006 - 2008

KOBAYASHI Ryoji, TOKUMITSU Hiorshi
Analysis of calmodulin-kinase cascade by using functional proteomics.

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, 2005 - 2006

TOKUMITSU Hiroshi, KOBAYASHI Ryoji, HATANO Naoya
Regeneration of pancreatic beta-cells that secret insulin in response to glucose stimulation

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, 2005 - 2006

ISHIDA Toshihiko, TOKUMITSU Hiroshi, MURAO Koji, OHNISHI Hiroaki, IMACHI Hitomi
Determine the molecular mechanism of insulin gene expression for the regeneration of pancreatic islets

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, 2003 - 2004

ISHIDA Toshihiko, MURAO Koji, TOKUMITSU Hiroshi, YOSHITAKA Sayo, OHNISHI Hiroaki
Physiological function of calcium/calmodulin-dependent protein kinase cascade

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, 2002 - 2003

TOKUMITSU Hiroshi, KOBAYASHI Ryoji, MURAO Koji, ISHIDA Toshihiko, SAJI Ikutaro
Study on structure biology and molecular pathology of Clostridium perfringens epsilon-toxin

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, 2001 - 2002

OKABE akinobu, KOBAYASHI ryoji, MIYATA shigeru, MATSUSHITA osamu, TOKUDA masaki, TOKUMITSU hiroshi
Regulation of gene expression mediated by Ca^<2+>/calmodulin-dependent protein kinase cascade

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, 2000 - 2001

TOKUMITSU Hiroshi, KIMURA Yoshishige, KOBAYASHI Ryoji, MURAO Koji
卵巣癌細胞における薬剤耐性克服薬の新規開発とその機能

科学研究費助成事業がん特別研究, がん特別研究, 日本学術振興会, 1992 - 1992

日高弘義, 徳光浩, 渡辺正人
The Development of the Strategy for the Presumption of the Tertiary Structure of Protein Kinases by Specific Inhibitors

Grants-in-Aid for Scientific Research Grant-in-Aid for Developmental Scientific Research (B), Grant-in-Aid for Developmental Scientific Research (B), Japan Society for the Promotion of Science, 1990 - 1992

HIDAKA Hiroyoshi, TOKUMITSU Hiroshi, WATANABE Masato, HAGIWARA Masatoshi, KOBAYASHI Ryoji
Unification and reconstruction of myosin phosphorylation theory on contractile response of smooth muscle and nonmuscle cells.

Grants-in-Aid for Scientific Research Grant-in-Aid for international Scientific Research, Grant-in-Aid for international Scientific Research, Japan Society for the Promotion of Science, 1989 - 1991

HIDAKA Hiroyoshi, HARTSHORNE David J., TOKUMITSU Hiroshi, WATANABE Masato, KOBAYASHI Ryoji

Academic Activities

日本生化学会評議員・代議員

Planning, management, etc.

日本生化学会, 2018 - Present
日本学術振興会特別研究員等審査会専門委員

Review, evaluation

日本学術振興会研究者養成課特別研究員等審査会, 2020 - 2021

Profile Information

Research Interests

Research Areas

Research History

Papers

Misc.

Books and Other Publications

Research Projects

Academic Activities

Menu

Coauthors