2013年7月
A new nonscanning confocal microscopy module for functional voltage-sensitive dye and Ca2+ imaging of neuronal circuit activity
JOURNAL OF NEUROPHYSIOLOGY
- ,
- 巻
- 110
- 号
- 2
- 開始ページ
- 553
- 終了ページ
- 561
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1152/jn.00856.2012
- 出版者・発行元
- AMER PHYSIOLOGICAL SOC
Recent advances in fluorescent confocal microscopy and voltage-sensitive and Ca2+ dyes have vastly improved our ability to image neuronal circuits. However, existing confocal systems are not fast enough or too noisy for many live-cell functional imaging studies. Here, we describe and demonstrate the function of a novel, nonscanning confocal microscopy module. The optics, which are designed to fit the standard camera port of the Olympus BX51WI epifluorescent microscope, achieve a high signal-to-noise ratio (SNR) at high temporal resolution, making this configuration ideal for functional imaging of neuronal activities such as the voltage-sensitive dye (VSD) imaging. The optics employ fixed 100- X 100-pinhole arrays at the back focal plane (optical conjugation plane), above the tube lens of a usual upright microscope. The excitation light travels through these pinholes, and the fluorescence signal, emitted from subject, passes through corresponding pinholes before exciting the photodiodes of the imager: a 100- X 100-pixel metal-oxide semiconductor (MOS)-type pixel imager with each pixel corresponding to a single 100- X 100- mu m photodiode. This design eliminated the need for a scanning device; therefore, acquisition rate of the imager (maximum rate of 10 kHz) is the only factor limiting acquisition speed. We tested the application of the system for VSD and Ca2+ imaging of evoked neuronal responses on electrical stimuli in rat hippocampal slices. The results indicate that, at least for these applications, the new microscope maintains a high SNR at image acquisition rates of <= 0.3 ms per frame.
- リンク情報
- ID情報
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- DOI : 10.1152/jn.00856.2012
- ISSN : 0022-3077
- PubMed ID : 23615547
- Web of Science ID : WOS:000321843800024