論文

査読有り
2017年4月

P2Y12 receptors in primary microglia activate nuclear factor of activated T-cell signaling to induce C-C chemokine 3 expression

JOURNAL OF NEUROCHEMISTRY
  • Hidetoshi Tozaki-Saitoh
  • ,
  • Hiroyuki Miyata
  • ,
  • Tomohiro Yamashita
  • ,
  • Katsuyuki Matsushita
  • ,
  • Makoto Tsuda
  • ,
  • Kazuhide Inoue

141
1
開始ページ
100
終了ページ
110
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/jnc.13968
出版者・発行元
WILEY

Microglia are widely accepted as surveillants in the central nervous system that are continually searching the local environment for signs of injury. Following an inflammatory situation, microglia alter their morphology, extend ramified processes, and undergo cell body hypertrophy. Extracellular nucleotides are recognized as a danger signal by microglia. ADP acting on P2Y12 receptors induce process extension of microglia thereby attracting microglia to the site of adenosine tri-phosphate/ADP leaking or release. However, the question whether ADP/P2Y12 receptor signaling directly stimulates the production or release of inducible factors such as cytokines remains unclear. In this study, we found that CC chemokine ligand 3 (CCL3) is induced by ADP-treated primary microglia. Pharmacological characterization using pertussis toxin, a P2Y12 receptor inhibitor, and a calcium chelator revealed that CCL3 induction was caused by P2Y12 receptor-mediated intracellular calcium elevation. Next, nuclear factor of activated T-cell dephosphorylation and nuclear translocalization were observed. Calcineurin, an inhibitor for nuclear factor of activated T cell, suppressed CCL3 induction. These data indicate that microglial P2Y12 receptors are utilized to trigger an acute inflammatory response in microglia via rapid CCL3 induction after ADP stimulation.

Web of Science ® 被引用回数 : 5

リンク情報
DOI
https://doi.org/10.1111/jnc.13968
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28144998
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000397502400009&DestApp=WOS_CPL

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