2020年11月2日
Optogenetic regulation of embryo implantation in mice using photoactivatable CRISPR-Cas9.
Proceedings of the National Academy of Sciences of the United States of America
- 巻
- 117
- 号
- 46
- 開始ページ
- 28579
- 終了ページ
- 28581
- 記述言語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1073/pnas.2016850117
- 出版者・発行元
- Proceedings of the National Academy of Sciences
Embryo implantation is achieved upon successful interaction between a fertilized egg and receptive endometrium and is mediated by spatiotemporal expression of implantation-associated molecules including leukemia inhibitory factor (LIF). Here we demonstrate, in mice, that LIF knockdown via a photoactivatable CRISPR-Cas9 gene editing system and illumination with a light-emitting diode can spatiotemporally disrupt fertility. This system enables dissection of spatiotemporal molecular mechanisms associated with embryo implantation and provides a therapeutic strategy for temporal control of reproductive functions in vivo.
- リンク情報
- ID情報
-
- DOI : 10.1073/pnas.2016850117
- ISSN : 0027-8424
- eISSN : 1091-6490
- ORCIDのPut Code : 90407520
- PubMed ID : 33139551
- PubMed Central 記事ID : PMC7682590