論文

査読有り
2015年11月

The power of single molecule real-time sequencing technology in the de novo assembly of a eukaryotic genome

SCIENTIFIC REPORTS
  • Hiroaki Sakai
  • Ken Naito
  • Eri Ogiso-Tanaka
  • Yu Takahashi
  • Kohtaro Iseki
  • Chiaki Muto
  • Kazuhito Satou
  • Kuniko Teruya
  • Akino Shiroma
  • Makiko Shimoji
  • Takashi Hirano
  • Takeshi Itoh
  • Akito Kaga
  • Norihiko Tomooka
  • 全て表示

5
開始ページ
16780
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/srep16780
出版者・発行元
NATURE PUBLISHING GROUP

Second-generation sequencers (SGS) have been game-changing, achieving cost-effective whole genome sequencing in many non-model organisms. However, a large portion of the genomes still remains unassembled. We reconstructed azuki bean (Vigna angularis) genome using single molecule real-time (SMRT) sequencing technology and achieved the best contiguity and coverage among currently assembled legume crops. The SMRT-based assembly produced 100 times longer contigs with 100 times smaller amount of gaps compared to the SGS-based assemblies. A detailed comparison between the assemblies revealed that the SMRT-based assembly enabled a more comprehensive gene annotation than the SGS-based assemblies where thousands of genes were missing or fragmented. A chromosome-scale assembly was generated based on the high-density genetic map, covering 86% of the azuki bean genome. We demonstrated that SMRT technology, though still needed support of SGS data, achieved a near-complete assembly of a eukaryotic genome.

リンク情報
DOI
https://doi.org/10.1038/srep16780
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26616024
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000365422000002&DestApp=WOS_CPL
ID情報
  • DOI : 10.1038/srep16780
  • ISSN : 2045-2322
  • PubMed ID : 26616024
  • Web of Science ID : WOS:000365422000002

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