2015年11月
The power of single molecule real-time sequencing technology in the de novo assembly of a eukaryotic genome
SCIENTIFIC REPORTS
- 巻
- 5
- 号
- 開始ページ
- 16780
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/srep16780
- 出版者・発行元
- NATURE PUBLISHING GROUP
Second-generation sequencers (SGS) have been game-changing, achieving cost-effective whole genome sequencing in many non-model organisms. However, a large portion of the genomes still remains unassembled. We reconstructed azuki bean (Vigna angularis) genome using single molecule real-time (SMRT) sequencing technology and achieved the best contiguity and coverage among currently assembled legume crops. The SMRT-based assembly produced 100 times longer contigs with 100 times smaller amount of gaps compared to the SGS-based assemblies. A detailed comparison between the assemblies revealed that the SMRT-based assembly enabled a more comprehensive gene annotation than the SGS-based assemblies where thousands of genes were missing or fragmented. A chromosome-scale assembly was generated based on the high-density genetic map, covering 86% of the azuki bean genome. We demonstrated that SMRT technology, though still needed support of SGS data, achieved a near-complete assembly of a eukaryotic genome.
- リンク情報
- ID情報
-
- DOI : 10.1038/srep16780
- ISSN : 2045-2322
- PubMed ID : 26616024
- Web of Science ID : WOS:000365422000002