Papers

Peer-reviewed Lead author Corresponding author
Feb, 2020

Metabolic Profiling of the Cerebrospinal Fluid in Pediatric Epilepsy.

Acta medica Okayama
  • Tomoyuki Akiyama
  • ,
  • Daisuke Saigusa
  • ,
  • Yuki Hyodo
  • ,
  • Keiko Umeda
  • ,
  • Reina Saijo
  • ,
  • Seizo Koshiba
  • ,
  • Katsuhiro Kobayashi

Volume
74
Number
1
First page
65
Last page
72
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.18926/AMO/57955

To characterize metabolic profiles within the central nervous system in epilepsy, we performed gas chromatography-tandem mass spectrometry (GC-MS/MS)-based metabolome analysis of the cerebrospinal fluid (CSF) in pediatric patients with and without epilepsy. The CSF samples obtained from 64 patients were analyzed by GC-MS/MS. Multivariate analyses were performed for two age groups, 0-5 years of age and 6-17 years of age, to elucidate the effects of epilepsy and antiepileptic drugs on the metabolites. In patients aged 0-5 years (22 patients with epilepsy, 13 without epilepsy), epilepsy patients had reduced 2-ketoglutaric acid and elevated pyridoxamine and tyrosine. In patients aged 6-17 years (12 with epilepsy, 17 without epilepsy), epilepsy patients had reduced 1,5-anhydroglucitol. Valproic acid was associated with elevated 2-aminobutyric acid, 2-ketoisocaproic acid, 4-hydroxyproline, acetylglycine, methionine, N-acetylserine, and serine. Reduced energy metabolism and alteration of vitamin B6 metabolism may play a role in epilepsy in young children. The roles of 1,5-anhydroglucitol in epilepsy in older children and in levetiracetam and zonisamide treatment remain to be explained. Valproic acid influenced the levels of amino acids and related metabolites involved in the metabolism of serine, methionine, and leucine.

Link information
DOI
https://doi.org/10.18926/AMO/57955
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32099251
ID information
  • DOI : 10.18926/AMO/57955
  • Pubmed ID : 32099251

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