論文

査読有り
2008年6月

Schizosaccharomyces pombe Snf2SR, a novel SNF2 family protein, interacts with Ran GTPase and modulates both RanGEF and RanGAP activities

GENES TO CELLS
  • Tomoyuki Ohba
  • ,
  • Hitoshi Nishijima
  • ,
  • Hideo Nishitani
  • ,
  • Takeharu Nishimoto

13
6
開始ページ
571
終了ページ
582
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/j.1365-2443.2008.01190.x
出版者・発行元
BLACKWELL PUBLISHING

Snf2SR, a suppressor of rna1(ts), which is a temperature-sensitive mutation in Schizosaccharomyces pombe RanGAP (GTPase activating protein), possesses both the SNF2 and the helicase domains conserved in the chromatin remodeling SNF2 ATPase/helicase protein family. We have now clarified a function of Snf2SR. Snf2SR indeed showed DNA-stimulated ATPase activity, proving that it is a member of the SNF2 ATPase/helicase family. Consistent with this role, Snf2SR was localized in the nucleus and cell fractionation analysis revealed that Snf2SR was tightly associated with the nuclear matrix. The disruption of snf2SR(+) was detrimental for a cell proliferation of S. pombe. Snf2SR that did not enhance RanGAP activity by itself, but abolished histone-H3-mediated RanGAP inhibition, as previously reported for the histone H3 methyltransferase, Clr4, another rna1(ts) suppressor. In contrast to Clr4, Snf2SR directly bound to the GDP-bound form of the S. pombe Ran homologue Spi1 and enhanced the nucleotide exchange activity of Pim1, the S. pombe RanGEF (guanine nucleotide exchange factor). Over-expression of Spi1-G18V, a Ran GTPase mutant fixed in the GTP-bound form, was lethal to S. pombe Delta snf2SR. Together, our results indicate that Snf2SR is involved in the Ran GTPase cycle in vivo.

Web of Science ® 被引用回数 : 3

リンク情報
DOI
https://doi.org/10.1111/j.1365-2443.2008.01190.x
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/18422602
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000256351200004&DestApp=WOS_CPL

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