2006年4月
Structural basis for RNA unwinding by the DEAD-box protein Drosophila vasa
CELL
- ,
- ,
- ,
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- 巻
- 125
- 号
- 2
- 開始ページ
- 287
- 終了ページ
- 300
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.cell.2006.01.054
- 出版者・発行元
- CELL PRESS
DEAD-box RNA helicases, which regulate various processes involving RNA, have two RecA-like domains as a catalytic core to alter higher-order RNA structures. We determined the 2.2 angstrom resolution structure of the core of the Drosophila DEAD-box protein Vasa in complex with a single-stranded RNA and an ATP analog. The ATP analog intensively interacts with both of the domains, thereby bringing them into the closed form, with many interdomain interactions of conserved residues. The bound RNA is sharply bent, avoiding a clash with a conserved alpha helix in the N-terminal domain. This "wedge" helix should disrupt base pairs by bending one of the strands when a duplex is bound. Mutational analyses indicated that the interdomain interactions couple ATP hydrolysis to RNA unwinding, probably through fine positioning of the duplex relative to the wedge helix. This mechanism, which differs from those for canonical translocating helicases, may enable the targeted modulation of intricate RNA structures.
- リンク情報
-
- DOI
- https://doi.org/10.1016/j.cell.2006.01.054
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902257847859809
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/16630817
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000237241500019&DestApp=WOS_CPL
- URL
- http://orcid.org/0000-0001-9461-8714
- ID情報
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- DOI : 10.1016/j.cell.2006.01.054
- ISSN : 0092-8674
- J-Global ID : 200902257847859809
- ORCIDのPut Code : 27338020
- PubMed ID : 16630817
- Web of Science ID : WOS:000237241500019