論文

国際誌
2021年3月1日

Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation.

Scientific reports
  • Toshihide Hamabe-Horiike
  • ,
  • Kanji Kawasaki
  • ,
  • Masataka Sakashita
  • ,
  • Chihiro Ishizu
  • ,
  • Tomokazu Yoshizaki
  • ,
  • Shin-Ichi Harada
  • ,
  • Keiko Ogawa-Ochiai
  • ,
  • Yohei Shinmyo
  • ,
  • Hiroshi Kawasaki

11
1
開始ページ
4864
終了ページ
4864
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41598-021-84210-z

Glial cells such as astrocytes and oligodendrocytes play crucial roles in the central nervous system. To investigate the molecular mechanisms underlying the development and the biological functions of glial cells, simple and rapid techniques for glial cell-specific genetic manipulation in the mouse cerebrum would be valuable. Here we uncovered that the Gfa2 promoter is suitable for selective gene expression in astrocytes when used with the piggyBac system and in utero electroporation. In contrast, the Blbp promoter, which has been used to induce astrocyte-specific gene expression in transgenic mice, did not result in astrocyte-specific gene expression. We also identified the Plp1 and Mbp promoters could be used with the piggyBac system and in utero electroporation to induce selective gene expression in oligodendrocytes. Furthermore, using our technique, neuron-astrocyte or neuron-oligodendrocyte interactions can be visualized by labeling neurons, astrocytes and oligodendrocytes differentially. Our study provides a fundamental basis for specific transgene expression in astrocytes and/or oligodendrocytes in the mouse cerebrum.

リンク情報
DOI
https://doi.org/10.1038/s41598-021-84210-z
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/33649472
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921133
ID情報
  • DOI : 10.1038/s41598-021-84210-z
  • PubMed ID : 33649472
  • PubMed Central 記事ID : PMC7921133

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