論文

査読有り 責任著者
2018年7月

A mutant HCN4 channel in a family with bradycardia, left bundle branch block, and left ventricular noncompaction.

Heart and Vessels
  • Ryosuke Yokoyama
  • Koshi Kinoshita
  • Yukiko Hata
  • Masayoshi Abe
  • Kenta Matsuoka
  • Keiichi Hirono
  • Masanobu Kano
  • Makoto Nakazawa
  • Fukiko Ichida
  • Naoki Nishida
  • Toshihide Tabata
  • 全て表示

33
7
開始ページ
802
終了ページ
819
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00380-018-1116-6

We found that a female infant presenting with left bundle branch block and left ventricular noncompaction carries uninvestigated gene mutations HCN4(G811E), SCN5A(L1988R), DMD(S2384Y), and EMD(R203H). Here, we explored the possible pathogenicity of HCN4(G811E), which results in a G811E substitution in hyperpolarization-activated cyclic nucleotide-gated channel 4, the main subunit of the cardiac pacemaker channel. Voltage-clamp measurements in a heterologous expression system of HEK293T cells showed that HCN4(G811E) slightly reduced whole-cell HCN4 channel conductance, whereas it did not affect the gating kinetics, unitary conductance, or cAMP-dependent modulation of voltage-dependence. Immunocytochemistry and immunoblot analysis showed that the G811E mutation did not impair the membrane trafficking of the channel subunit in the heterologous expression system. These findings indicate that HCN4(G811E) may not be a monogenic factor to cause the cardiac disorders.

リンク情報
DOI
https://doi.org/10.1007/s00380-018-1116-6
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29349559
ID情報
  • DOI : 10.1007/s00380-018-1116-6
  • PubMed ID : 29349559

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