論文

査読有り 国際誌
2014年5月14日

Evidence that dendritic mitochondria negatively regulate dendritic branching in pyramidal neurons in the neocortex.

The Journal of neuroscience : the official journal of the Society for Neuroscience
  • Toshiya Kimura
  • ,
  • Fujio Murakami

34
20
開始ページ
6938
終了ページ
51
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1523/JNEUROSCI.5095-13.2014
出版者・発行元
SOC NEUROSCIENCE

The precise branching patterns of dendritic arbors have a profound impact on information processing in individual neurons and the brain. These patterns are established by positive and negative regulation of the dendritic branching. Although the mechanisms for positive regulation have been extensively investigated, little is known about those for negative regulation. Here, we present evidence that mitochondria located in developing dendrites are involved in the negative regulation of dendritic branching. We visualized mitochondria in pyramidal neurons of the mouse neocortex during dendritic morphogenesis using in utero electroporation of a mitochondria-targeted fluorescent construct. We altered the mitochondrial distribution in vivo by overexpressing Mfn1, a mitochondrial shaping protein, or the Miro-binding domain of TRAK2 (TRAK2-MBD), a truncated form of a motor-adaptor protein. We found that dendritic mitochondria were preferentially targeted to the proximal portion of dendrites only during dendritic morphogenesis. Overexpression of Mfn1 or TRAK2-MBD depleted mitochondria from the dendrites, an effect that was accompanied by increased branching of the proximal portion of the dendrites. This dendritic abnormality cannot be accounted for by changes in the distribution of membrane trafficking organelles since the overexpression of Mfn1 did not alter the distributions of the endoplasmic reticulum, Golgi, or endosomes. Additionally, neither did these constructs impair neuronal viability or mitochondrial function. Therefore, our results suggest that dendritic mitochondria play a critical role in the establishment of the precise branching pattern of dendritic arbors by negatively affecting dendritic branching.

Web of Science ® 被引用回数 : 23

リンク情報
DOI
https://doi.org/10.1523/JNEUROSCI.5095-13.2014
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24828647
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6608104
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000336895000021&DestApp=WOS_CPL
ID情報
  • DOI : 10.1523/JNEUROSCI.5095-13.2014
  • ISSN : 0270-6474
  • PubMed ID : 24828647
  • PubMed Central 記事ID : PMC6608104
  • Web of Science ID : WOS:000336895000021

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