論文

査読有り
2012年4月

Intrinsic and Extrinsic Mechanisms Control the Termination of Cortical Interneuron Migration

JOURNAL OF NEUROSCIENCE
  • Naoko Inamura
  • ,
  • Toshiya Kimura
  • ,
  • Satoshi Tada
  • ,
  • Takashi Kurahashi
  • ,
  • Mitsutoshi Yanagida
  • ,
  • Yuchio Yanagawa
  • ,
  • Kazuhiro Ikenaka
  • ,
  • Fujio Murakami

32
17
開始ページ
6032
終了ページ
6042
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1523/JNEUROSCI.3446-11.2012
出版者・発行元
SOC NEUROSCIENCE

During development, neurons migrate from their site of origin to their final destinations. Upon reaching this destination, the termination of their migration is crucial for building functional architectures such as laminated structures and nuclei. How this termination is regulated, however, is not clear. Here, we investigated the contribution of cell-intrinsic mechanisms and extrinsic factors. Using GAD67-GFP knock-in mice and in utero electroporation cell labeling, we visualized GABAergic neurons and analyzed their motility in vitro. We find that the motility of GABAergic neurons in cortical slices gradually decreases as development proceeds and is almost abolished by the end of the first postnatal week. Consistent with this, a reduction of embryonic interneuron motility occurred in dissociated cultures. This is in part due to cell-intrinsic mechanisms, as a reduction in motility is observed during long-term culturing on glial feeder cells. Cell-intrinsic regulation is further supported by observations that interneurons labeled in early stages migrated more actively than those labeled in late stages in the same cortical explant. We found evidence suggesting that upregulation of the potassium-chloride cotransporter KCC2 underlies this intrinsic regulation. Reduced motility is also observed when embryonic interneurons are plated on postnatal cortical feeder cells, suggesting extrinsic factors derived from the postnatal cortex too contribute to termination. These factors should include secreted molecules, as cultured postnatal cortical cells could exercise this effect without directly contacting the interneuron. These findings suggest that intrinsic mechanisms and extrinsic factors coordinate to reduce the motility of migrating neurons, thereby leading to the termination of migration.

Web of Science ® 被引用回数 : 20

リンク情報
DOI
https://doi.org/10.1523/JNEUROSCI.3446-11.2012
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000303393000032&DestApp=WOS_CPL
ID情報
  • DOI : 10.1523/JNEUROSCI.3446-11.2012
  • ISSN : 0270-6474
  • Web of Science ID : WOS:000303393000032

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