論文

国際誌
2021年2月5日

CAMSAP3 is required for mTORC1-dependent ependymal cell growth and lateral ventricle shaping in mouse brains.

Development (Cambridge, England)
  • Toshiya Kimura
  • ,
  • Hiroko Saito
  • ,
  • Miwa Kawasaki
  • ,
  • Masatoshi Takeichi

148
3
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1242/dev.195073

Microtubules (MTs) regulate numerous cellular processes, but their roles in brain morphogenesis are not well known. Here, we show that CAMSAP3, a non-centrosomal microtubule regulator, is important for shaping the lateral ventricles. In differentiating ependymal cells, CAMSAP3 became concentrated at the apical domains, serving to generate MT networks at these sites. Camsap3-mutated mice showed abnormally narrow lateral ventricles, in which excessive stenosis or fusion was induced, leading to a decrease of neural stem cells at the ventricular and subventricular zones. This defect was ascribed at least in part to a failure of neocortical ependymal cells to broaden their apical domain, a process necessary for expanding the ventricular cavities. mTORC1 was required for ependymal cell growth but its activity was downregulated in mutant cells. Lysosomes, which mediate mTORC1 activation, tended to be reduced at the apical regions of the mutant cells, along with disorganized apical MT networks at the corresponding sites. These findings suggest that CAMSAP3 supports mTORC1 signaling required for ependymal cell growth via MT network regulation, and, in turn, shaping of the lateral ventricles.

リンク情報
DOI
https://doi.org/10.1242/dev.195073
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/33462112
ID情報
  • DOI : 10.1242/dev.195073
  • PubMed ID : 33462112

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