論文

査読有り 最終著者
2008年10月

Construction of a BAC library for buckwheat genome research - An application to positional cloning of agriculturally valuable traits

GENES & GENETIC SYSTEMS
  • Yasuo Yasui
  • ,
  • Masashi Mori
  • ,
  • Daiki Matsumoto
  • ,
  • Ohmi Ohnishi
  • ,
  • Clayton G. Campbell
  • ,
  • Tatsuya Ota

83
5
開始ページ
393
終了ページ
401
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1266/ggs.83.393
出版者・発行元
GENETICS SOC JAPAN

We have constructed a BAC library for common buckwheat Fagopyrum esculentum Moench. The library includes 142,005 clones with an average insert size of similar to 76 kb equivalent to approximately a 7 similar to 8-fold coverage of the genome. Polymerase chain reaction based screening of the library with AGAMOUS and FLORICAULA/LEAFY primers, has identified 7 and 9 BACs, respectively, which are consistent with the genome coverage. This library represents the first large insert genomic library for F. esculentum and it can be served as a genetic resource facilitating agricultural, pharmacological, physiological, and evolutionary studies of the species. To demonstrate the utilization of the library for characterizing agriculturally valuable traits, we developed a sequence tagged site marker tightly linked to the dwarf E locus as well as to the self-incompatibility complex locus and screened the library to initiate positional cloning of the causative genes.

リンク情報
DOI
https://doi.org/10.1266/ggs.83.393
CiNii Articles
http://ci.nii.ac.jp/naid/10023887944
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000263862400004&DestApp=WOS_CPL
ID情報
  • DOI : 10.1266/ggs.83.393
  • ISSN : 1341-7568
  • eISSN : 1880-5779
  • CiNii Articles ID : 10023887944
  • Web of Science ID : WOS:000263862400004

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