2008年10月
Construction of a BAC library for buckwheat genome research - An application to positional cloning of agriculturally valuable traits
GENES & GENETIC SYSTEMS
- ,
- ,
- ,
- ,
- ,
- 巻
- 83
- 号
- 5
- 開始ページ
- 393
- 終了ページ
- 401
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1266/ggs.83.393
- 出版者・発行元
- GENETICS SOC JAPAN
We have constructed a BAC library for common buckwheat Fagopyrum esculentum Moench. The library includes 142,005 clones with an average insert size of similar to 76 kb equivalent to approximately a 7 similar to 8-fold coverage of the genome. Polymerase chain reaction based screening of the library with AGAMOUS and FLORICAULA/LEAFY primers, has identified 7 and 9 BACs, respectively, which are consistent with the genome coverage. This library represents the first large insert genomic library for F. esculentum and it can be served as a genetic resource facilitating agricultural, pharmacological, physiological, and evolutionary studies of the species. To demonstrate the utilization of the library for characterizing agriculturally valuable traits, we developed a sequence tagged site marker tightly linked to the dwarf E locus as well as to the self-incompatibility complex locus and screened the library to initiate positional cloning of the causative genes.
- リンク情報
- ID情報
-
- DOI : 10.1266/ggs.83.393
- ISSN : 1341-7568
- eISSN : 1880-5779
- CiNii Articles ID : 10023887944
- Web of Science ID : WOS:000263862400004