2008年9月
Zebrafish mutants with disrupted early T-cell and thymus development identified in early pressure screen
DEVELOPMENTAL DYNAMICS
- 巻
- 237
- 号
- 9
- 開始ページ
- 2575
- 終了ページ
- 2584
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/dvdy.21683
- 出版者・発行元
- WILEY-LISS
Generation of mature T lymphocytes requires an intact hematopoietic stem cell compartment and functional thymic epithelium. We used the zebrafish (Danio rerio) to isolate mutations that affect the earliest steps in T lymphopoiesis and thymic organogenesis. Here we describe the results of a genetic screen in which gynogenetic diploid offspring from heterozygous females were analyzed by whole-mount in situ hybridization for the expression of rag-1. To assess immediately if a global defect in hematopoiesis resulted in the mutant phenotype, alpha-embryonic globin expression was simultaneously assayed for multilineage defects. In this report, we present the results obtained with this strategy and show representative mutant phenotypes affecting early steps in T-cell development and/or thymic epithelial cell development. We discuss the advantage of this strategy and the general usefulness of the zebrafish as a model system for vertebrate lymphopoiesis and thymic organogenesis.
- リンク情報
- ID情報
-
- DOI : 10.1002/dvdy.21683
- ISSN : 1058-8388
- Web of Science ID : WOS:000259289800029