論文

査読有り 国際共著 国際誌
2008年9月

Zebrafish mutants with disrupted early T-cell and thymus development identified in early pressure screen

DEVELOPMENTAL DYNAMICS
  • Nikolaus S. Trede
  • Tatsuya Ota
  • Hirohide Kawasaki
  • Barry H. Paw
  • Tammisty Katz
  • Bradley Demarest
  • Sarah Hutchinson
  • Yi Zhou
  • Candace Hersey
  • Agustin Zapata
  • Chris T. Amemiya
  • Leonard I. Zon
  • 全て表示

237
9
開始ページ
2575
終了ページ
2584
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/dvdy.21683
出版者・発行元
WILEY-LISS

Generation of mature T lymphocytes requires an intact hematopoietic stem cell compartment and functional thymic epithelium. We used the zebrafish (Danio rerio) to isolate mutations that affect the earliest steps in T lymphopoiesis and thymic organogenesis. Here we describe the results of a genetic screen in which gynogenetic diploid offspring from heterozygous females were analyzed by whole-mount in situ hybridization for the expression of rag-1. To assess immediately if a global defect in hematopoiesis resulted in the mutant phenotype, alpha-embryonic globin expression was simultaneously assayed for multilineage defects. In this report, we present the results obtained with this strategy and show representative mutant phenotypes affecting early steps in T-cell development and/or thymic epithelial cell development. We discuss the advantage of this strategy and the general usefulness of the zebrafish as a model system for vertebrate lymphopoiesis and thymic organogenesis.

リンク情報
DOI
https://doi.org/10.1002/dvdy.21683
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000259289800029&DestApp=WOS_CPL
ID情報
  • DOI : 10.1002/dvdy.21683
  • ISSN : 1058-8388
  • Web of Science ID : WOS:000259289800029

エクスポート
BibTeX RIS