2004年5月
Evaluation of disinfective potential of reactivated free chlorine in pooled tap water by electrolysis
JOURNAL OF MICROBIOLOGICAL METHODS
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- 巻
- 57
- 号
- 2
- 開始ページ
- 163
- 終了ページ
- 173
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1016/j.mimet.2003.12.011
- 出版者・発行元
- ELSEVIER SCIENCE BV
Tap water is one of the causative factors of hospital infections. We examined the disinfective potential of electrolysis and mechanism of disinfection, and clarified the disinfective effect of electrolysis on tap water contaminated with bacteria, and discussed its clinical applications. Tap waters artificially contaminated with Pseudomonas aeruginosa, Escherichia coli, Legionella pneumophila, and Staphylococcus aureus could be sterilized by electrolysis at 20-30 mA for 5 min. A high-density suspension (10(6) CFU/ml) of a spore forming bacterium, Bacillus subtilis was not completely sterilized by electrolysis at 50 mA it]) to 30 train, but a low-density suspension (105 CFU/ml) was totally sterilized by electrolysis at 50 mA for 5 trill. Electrolyzed P aeruginosa changed morphologically, that is, there was bleb formation on the cell wall and irregular aggregation of cytoplasmic small granules. Moreover, cytoplasmic enzyme, nitrate reductase, was inactivated by the electrolysis. On the other hand. genomic DNA of the electrolyzed bacteria was not degenerated, therefore, their DNA polymerase activity was not completely inactivated. Consequently, the major agent in electrolysis for bactericidal action was considered to be free chlorine, and the possible bactericidal mechanism was by destruction of bacterial membranes, followed by the aggregation of peripheral cytoplasmic proteins. Electrolysis of tap water for both disinfecting contaminating bacteria and increasing the disinfectant capacity was considered effective with some limitations, particularly against high-density contamination by spore-forming bacteria. In clinical settings, electrolysis of tap water is considered effective to disinfect water for hand washing in operation theatres. and bathing water for immunocompromised hosts. (C) 2004 Elsevier B.V. All rights reserved.
- リンク情報
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- DOI
- https://doi.org/10.1016/j.mimet.2003.12.011
- CiNii Articles
- http://ci.nii.ac.jp/naid/80016593679
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/15063056
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000220936000003&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1016/j.mimet.2003.12.011
- ISSN : 0167-7012
- CiNii Articles ID : 80016593679
- PubMed ID : 15063056
- Web of Science ID : WOS:000220936000003