2018年8月24日
Chemical Landscape for Tissue Clearing based on Hydrophilic Reagents
Cell Reports
- 巻
- 24
- 号
- 8
- 開始ページ
- 2196
- 終了ページ
- 2210
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.celrep.2018.07.056.
- 出版者・発行元
- Cell Press
We describe a strategy for developing hydrophilic chemical cocktails for tissue delipidation, decoloring, refractive index (RI) matching, and decalcification, based on comprehensive chemical profiling. More than 1,600 chemicals were screened by a high-throughput evaluation system for each chemical process. The chemical profiling revealed important chemical factors: salt-free amine with high octanol/water partition-coefficient (logP) for delipidation, N-alkylimidazole for decoloring, aromatic amide for RI matching, and protonation of phosphate ion for decalcification. The strategic integration of optimal chemical cocktails provided a series of CUBIC (clear, unobstructed brain/body imaging cocktails and computational analysis) protocols, which efficiently clear mouse organs, mouse body including bone, and even large primate and human tissues. The updated CUBIC protocols are scalable and reproducible, and they enable three-dimensional imaging of the mammalian body and large primate and human tissues. This strategy represents a future paradigm for the rational design of hydrophilic clearing cocktails that can be used for large tissues.
- リンク情報
-
- DOI
- https://doi.org/10.1016/j.celrep.2018.07.056.
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/30134179
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000442693600025&DestApp=WOS_CPL
- URL
- https://www.sciencedirect.com/science/article/pii/S2211124718311598?via%3Dihub
- ID情報
-
- DOI : 10.1016/j.celrep.2018.07.056.
- ORCIDのPut Code : 48889598
- PubMed ID : 30134179
- Web of Science ID : WOS:000442693600025